关键词: 鼠疫耶尔森菌, 生化特征, 西藏自治区

Abstract:

Objective To understand the mutations of Yersinia pestis, all of strains isolated in Tibet during 2009 to 2011 were selected to identify the biochemical features of the pathogen. Methods Traditional methods like glycerol fermentation, glucose metabolism and nitrate reduction were carried out. Tested strains isolated from Tibet during the year of 2009 and 2011, were incubated in biochemical action tubes made recently in 28℃ for 14 d, and the color of every tubes was observed and recorded each day. Results All of 111 tested strains were able to reduce nitrate and ferment glycerol, but unable to catabolize rhamnose; Nine of tested strains were capable of catabolizing arabinose, six of them isolated from Naqu and the other three from southern valley of the Qinghai-Tibet Plateau; The results of arabinose metabolism of the other 102 strains were all negative. Conclusion It was suggested that all tested strains belong to Biovar Antiqua. Those Y. pestis strains isolated from Naqu located in the northern Tibet were Qinghai-Tibet Plateau Ecotype, and most strains isolated from southern valley of the Qinghai-Tibet Plateau were Gangdise Mountain Ecotype except only three strains, which had the same biochemical features as the strains of Qinghai-Tibet Plateau Ecotype. It needs to utilize molecular methods through analyzing genetic markers to type the three special Y. pestis strains.

Key words: Yersinia pestis, Biochemical characteristic, Tibet