中国媒介生物学及控制杂志 ›› 2017, Vol. 28 ›› Issue (1): 31-34.DOI: 10.11853/j.issn.1003.8280.2017.01.009

• 论著 • 上一篇    下一篇

江苏口岸褐家鼠线粒体DNA细胞色素C氧化酶亚基Ⅰ基因的遗传多态性研究

胡双双1, 吴炳耀2, 何江2, 陆永昌3, 杨庆贵2, 陈峰4, 孙立新2   

  1. 1 安徽理工大学医学院, 安徽 淮南 232001;
    2 江苏国际旅行卫生保健中心, 南京 210019;
    3 江苏出入境检验检疫局, 南京 210001;
    4 南通出入境检验检疫局, 江苏 南通 226000
  • 收稿日期:2016-09-27 出版日期:2017-02-20 发布日期:2017-02-20
  • 通讯作者: 孙立新,Email:slxdt63@163.com
  • 作者简介:胡双双,女,在读硕士,主要从事生物医学工程专业及分子生物学研究,Email:gnauhshu@163.com
  • 基金资助:

    国家质检总局科研基金项目(2006IK130)

Research of the cytochrome C oxidase subunitⅠ gene polymorphisms for Rattus norvegicus in Jiangsu port

HU Shuang-shuang1, WU Bing-yao2, HE Jiang2, LU Yong-chang3, YANG Qing-gui2, CHEN Feng4, SUN Li-xin2   

  1. 1 School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China;
    2 Jiangsu International Travel Healthcare Center;
    3 Jiangsu Entry-Exit Inspection and Quarantine Bureau;
    4 Nantong Entry-Exit Inspection and Quarantine Bureau
  • Received:2016-09-27 Online:2017-02-20 Published:2017-02-20
  • Supported by:

    Supported by the Science and Technology Project of General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China(No. 2006IK130)

摘要:

目的 研究褐家鼠线粒体DNA细胞色素C氧化酶亚基Ⅰ(COⅠ)基因的遗传多态性特征,为国境口岸鼠形动物的鉴定和溯源提供技术支持。方法 通过对江苏口岸本底及输入性褐家鼠COⅠ基因的扩增、测序和比对,结合GenBank的褐家鼠信息,对褐家鼠进行遗传多态性分析和系统发育树研究。结果 获得48个国内外褐家鼠COⅠ基因序列,测出的605个序列位点中保守位点587个,可变异位点18个,核苷酸平均差异数为(2.510 000±0.000 640);江苏口岸本底褐家鼠仅为1个单倍型,非江苏口岸褐家鼠多态性丰富。结论 不同地区的褐家鼠具有一定的种内差异,该差异与地理位置分布有一定的关系。

关键词: 褐家鼠, 细胞色素C氧化酶亚基Ⅰ基因, 基因多样性, 地理分布

Abstract:

Objective The cytochrome C oxidase subunitⅠ(COⅠ) gene polymorphisms for Rattus norvegicus, was studied to provide support for the species identification of rodents from frontier port. Methods The COⅠ gene of R. norvegicus was amplified, sequenced, aligned and compared with the information on GenBank to analyze genetic polymorphism and create phylogenetic tree for R. norvegicus. Results Forty-eight COⅠ gene sequences were obtained from R. norvegicus. In this research, 605 loci were measured in each sequence, there were 587 conservative loci and 18 mutation loci, the nucleotide differences number was (2.510 000±0.000 640) on average. All of R. norvegicus in Jiangsu port background only shared one haploid type, other R. norvegicus' nucleotide polymorphism was rich. Conclusion There are species differences of the R. norvegicus in different areas, this kind of difference is related to the geographical origin.

Key words: Rattus norvegicus, Cytochrome C oxidase subunitⅠgene, Genetic diversity, Geographical distribution

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