中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (5): 470-473.DOI: 10.11853/j.issn.1003.8280.2016.05.012

• 论著 • 上一篇    下一篇

河北省116株鼠疫耶尔森菌差异片段基因分型及流行病学分析

王海峰, 刘合智, 白雪薇, 杨顺林, 周松, 胡乐乐, 刘溢洋, 李玉贵, 史献明   

  1. 河北省鼠疫防治所检验科, 河北 张家口 075000
  • 收稿日期:2016-04-11 出版日期:2016-10-20 发布日期:2016-10-20
  • 作者简介:王海峰,女,硕士,中级检验师,从事鼠疫检验研究,Email:whf584521@163.com
  • 基金资助:

    河北省2014年度医学科学研究重点课题计划(ZL20140119)

Genotyping of Yersinia pestis by different regions and its epidemiological characteristics in Hebei province

WANG Hai-feng, LIU He-zhi, BAI Xue-wei, YANG Shun-lin, ZHOU Song, HU Le-le, LIU Yi-yang, LI Yu-gui, SHI Xian-ming   

  1. Anti-plague Institute of Hebei Province, Zhangjiakou 075000, Hebei Province, China
  • Received:2016-04-11 Online:2016-10-20 Published:2016-10-20
  • Supported by:

    Supported by the Medical Science Research Project in Hebei Province (No. ZL20140119)

摘要:

目的 研究河北省鼠疫疫源地鼠疫耶尔森菌(鼠疫菌)菌株的基因型别和遗传特征。方法 根据鼠疫基因组的22个差异区段和pMT1设计引物,对河北省鼠疫疫源地分离的116株菌株进行分析。结果 116株鼠疫菌株均缺乏DFR01、DFR06、DFR07、DFR13、DFR15~18片段,参考DFR基因分型系统,河北省鼠疫疫源地的菌株为基因17型,主要分布于内蒙古自治区化德县、白旗、太仆寺旗相邻的康保县北部。结论 河北省鼠疫菌菌株遗传稳定,仅有1个基因分型,疫情流行趋于平缓。

关键词: 鼠疫耶尔森菌, 差异区段, 基因分型

Abstract:

Objective To study the genotype and epidemiological characteristics of Yersinia pestis in Hebei foci. Methods Primers were designed according to the confirmed 22 different segments (DFR), to genotype the 116 Y. pestis DNA of Hebei province. Results All of the strains lack these DFR of 01, 06, 07, 13, 15-18, to compare the genetic typing system of DFR, Y. pestis from Hebei province belong to the 17 genotype, distributed in the northern Kangbao county neighboring Huade, Baiqi, and Taipusiqi in the Inner Mongolia Autonomous Region. Conclusion The Y. pestis is stable genetically in Hebei plague foci, and have only one genotype, so the epidemic tends to stabilize.

Key words: Yersinia pestis, Different segments, Genetic typing

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