长角血蜱细菌群落结构及多样性研究

doi:10.11853/j.issn.1003.8280.2016.05.002

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (5): 426-431.DOI: 10.11853/j.issn.1003.8280.2016.05.002

长角血蜱细菌群落结构及多样性研究

韩娜^1,2, 张琳^1,2, 张雯^1,2, 强裕俊^1,2, 侯学霞^1,2, 陈晨³, 郝琴^1,2, 张媛媛³

1 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 北京 102206;
2 感染性疾病诊治协同创新中心, 浙江杭州 310003;
3 首都医科大学附属北京地坛医院传染病研究所, 新发突发传染病研究北京市重点实验室, 北京 100015

收稿日期:2016-05-23 出版日期:2016-10-20 发布日期:2016-10-20
通讯作者: 张媛媛,Email:lindazhangyy@126.com;郝琴,Email:haoqin@icdc.cn
作者简介:韩娜,女,博士,助理研究员,主要从事临床标本微生物群落结构和多样性研究,Email:hanna@icdc.cn;张琳,女,博士,助理研究员,从事莱姆病检测技术与空间生态学研究,Email:zhanglin@icdc.cn
基金资助:
国家自然科学基金青年基金（81301402）；国家高技术研究发展计划（863计划）（2014AA021505）

Studies on the composition and diversity of the bacterial community in Haemaphysalis longicornis

HAN Na^1,2, ZHANG Lin^1,2, ZHANG Wen^1,2, QIANG Yu-jun^1,2, HOU Xue-xia^1,2, CHEN Chen³, HAO Qin^1,2, ZHANG Yuan-yuan³

1 State Key Laboratory of Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases;
3 Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing Key Laboratory of Emerging Infectious Diseases

Received:2016-05-23 Online:2016-10-20 Published:2016-10-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81301402) and the National High Technology Research and Development Program of China (863 Program) (No. 2014AA021505)

摘要/Abstract

摘要：

目的了解长角血蜱细菌的群落结构及种群多样性。方法应用Illumina Miseq PE300二代测序技术测定长角血蜱细菌16S rDNA基因的V3～V4高变异区段序列，分析其细菌的群落结构、种群多样性、优势菌群以及常见病原菌的分布。结果获得用于分析的序列和OTU数目分别为1 070 624条和145 479个；香农多样性指数的稀疏曲线接近平台期，说明此次测序的深度合适，可充分代表长角血蜱细菌的实际种群结构。长角血蜱微生物优势菌群依次为变形菌门、厚壁菌门、放线菌门和拟杆菌门，其中以γ-变形菌纲相对丰度最高。长角血蜱的“核心微生物群”，聚集成2个“蜱簇”，GⅠ和GⅡ簇分别有21和11个特有微生物群。发现菌群中存在大量致病菌和条件致病菌，但未检测到螺旋体和支原体。结论野外长角血蜱携带的微生物物种组成和丰度均表现了较大差异性。

关键词: 长角血蜱, 细菌群落结构, 多样性, 二代测序

Abstract:

Objective To investigate the bacterial community composition and diversity in Haemaphysalis longicornis. Methods The metagenomic 16S rDNA V3-V4 profiling of bacteria were sequenced by Illumina Miseq PE300 sequencing platform to describe the bacterial community in H. longicornis ticks, to evaluate bacterial population diversity and to identify the predominant microorganisms and distribution of pathogens. Results A total of 1 070 624 Tags and 145 479 OTUs were obtained for the study. Rarefaction analysis showed a rare factional Shannon-Wiener index reaching a plateau, which indicated that sufficient numbers of sequences had been sequenced for diversity analyses and the richness of bacteria of H. longicornis. The predominated phyla in the H. longicornis were Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes sequentially. Gammaproteobacteria class showed the highest relative abundance. Also we identified a core bacterial community and divided the ticks into two groups. The 21 and 11 unique bacterial communities were detected in individual group, GⅠ and GⅡ respectively. A large number of pathogenic bacteria and opportunistic pathogens were found, there were no Spirochaeta and Mycoplasma present in the tick samples. Conclusion The bacterial community structure and distribution of pathogens obtained from H. longicornis ticks showed a high diversity.

Key words: Haemaphysalis longicornis, Bacterial community structure, Diversity, Next-generation sequencing

中图分类号:

R384.4

韩娜, 张琳, 张雯, 强裕俊, 侯学霞, 陈晨, 郝琴, 张媛媛. 长角血蜱细菌群落结构及多样性研究[J]. 中国媒介生物学及控制杂志, 2016, 27(5): 426-431.

HAN Na, ZHANG Lin, ZHANG Wen, QIANG Yu-jun, HOU Xue-xia, CHEN Chen, HAO Qin, ZHANG Yuan-yuan. Studies on the composition and diversity of the bacterial community in Haemaphysalis longicornis[J]. Chines Journal of Vector Biology and Control, 2016, 27(5): 426-431.

参考文献

[1] Jongejan F, Uilenberg G. The global importance of ticks[J]. Parasitology,2004,129 Suppl:S3-14.
[2] Yu ZJ, Wang H, Wang TH, et al. Tick-borne pathogens and the vector potential of ticks in China[J]. Parasit Vectors,2015,8(1): 24.
[3] Manichanh C, Chapple CE, Frangeul L, et al. A comparison of random sequence reads versus 16S rDNA sequences for estimating the biodiversity of a metagenomic library[J]. Nucl Acids Res,2008,36(16):5180-5188.
[4] Benson MJ, Gawronski JD, Eveleigh DE, et al. Intracellular symbionts and other bacteria associated with deer ticks (Ixodes scapularis) from Nantucket and Wellfleet, Cape Cod, Massachusetts[J]. Appl Environ Microbiol,2004,70(1): 616-620.
[5] Lalzar I, Harrus S, Mumcuoglu KY, et al. Composition and seasonal variation of Rhipicephalus turanicus and Rhipicephalus sanguineus bacterial communities[J]. Appl Environ Microbiol, 2012,78(12):4110-4116.
[6] Corby-Harris V, Pontaroli AC, Shimkets LJ, et al. Geographical distribution and diversity of bacteria associated with natural populations of Drosophila melanogaster[J]. Appl Environ Microbiol,2007,73(11):3470-3479.
[7] Gupta AK, Nayduch D, Verma P, et al. Phylogenetic characterization of bacteria in the gut of house flies (Musca domestica L.) [J]. FEMS Microbiol Ecol,2012,79(3): 581-593.
[8] Campbell CL, Mummey DL, Schmidtmann ET, et al. Culture-independent analysis of midgut microbiota in the arbovirus vector Culicoides sonorensis (Diptera: Ceratopogonidae) [J]. J Med Entomol,2004,41(3):340-348.
[9] 布坎南. 伯杰细菌鉴定手册[M]. 8版. 中国科学院微生物研究所,译. 北京:科学出版社,1984:729-735.
[10] Sears CL. A dynamic partnership: celebrating our gut flora[J]. Anaerobe,2005,11(5):247-251.
[11] 唐昊,赵瑜,廖芷卉,等. 微小牛蜱中肠内容物菌群结构分析[J]. 中国病原生物学杂志,2015,10(6):487-490.
[12] Han XY, Andrade RA. Brevundimonas diminuta infections and its resistance to fluoroquinolones[J]. J Antimicrob Chemother, 2005,55(6):853-859.

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长角血蜱细菌群落结构及多样性研究

Studies on the composition and diversity of the bacterial community in Haemaphysalis longicornis

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