逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染调查

doi:10.11853/j.issn.1003.8280.2016.04.007

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (4): 341-344.DOI: 10.11853/j.issn.1003.8280.2016.04.007

逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染调查

杨军¹, 王艳梅², 鞠文东², 焦丹³, 徐宁², 梁慧杰⁴, 王晓杰⁵, 付维明², 耿聪², 程成²

1 逊克出入境检验检疫局, 黑龙江逊克 164400;
2 黑龙江国际旅行卫生保健中心, 哈尔滨 150001;
3 黑河出入境检验检疫局, 黑龙江黑河 164300;
4 萝北出入境检验检疫局, 黑龙江萝北 154201;
5 黑龙江省皮肤病防治研究所, 哈尔滨 150001

收稿日期:2016-04-12 出版日期:2016-08-20 发布日期:2016-08-20
通讯作者: 程成,Email:ate_0428@126.com
作者简介:杨军,男,副主任医师,主要从事国境卫生检疫工作,Email:yangjun4453194@163.com
基金资助:
黑龙江出入境检验检疫局科技计划项目（2015HK004）

Investigation on emerging tick-borne pathogens and co-infection in ticks in lava area of Xunke county, Heilongjiang province

YANG Jun¹, WANG Yan-mei², JU Wen-dong², JIAO Dan³, XU Ning², LIANG Hui-jie⁴, WANG Xiao-jie⁵, FU Wei-ming², GENG Cong², CHENG Cheng²

1 Xunke Entry-Exit Inspection and Quarantine Bureau, Xunke 164400, Heilongjiang Province, China;
2 Heilongjiang International Travel Healthcare Center;
3 Heihe Entry-Exit Inspection and Quarantine Bureau;
4 Luobei Entry-Exit Inspection and Quarantine Bureau;
5 Heilongjiang Institute of Dermatology Prevention

Received:2016-04-12 Online:2016-08-20 Published:2016-08-20
Supported by:
Supported by the Project Received the Support from Heilongjiang Entry-Exit Inspection and Quarantine Bureau (No. 2015HK004)

摘要/Abstract

摘要：

目的调查黑龙江省逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染情况。方法 2015年4－6月采集活蜱样本257只，利用PCR方法分别扩增6种新发蜱传病原体的特异性核酸片段，通过基因测序进一步鉴定。结果共检出斑点热群立克次体、巴尔通体和嗜吞噬细胞无形体核酸阳性样本分别为86、5和11例，感染率分别为33.46%、1.94%和4.28%。伯氏疏螺旋体、巴贝西虫和埃立克体的DNA核酸检测结果均为阴性。森林革蜱、嗜群血蜱和全沟硬蜱体内共检出复合感染8例，感染率为3.11%，证实该地区森林革蜱中存在斑点热群立克次体及巴尔通体，嗜群血蜱和全沟硬蜱存在斑点热群立克次体及嗜吞噬细胞无形体复合感染的情况。结论应加强黑龙江省逊克县火山熔岩地区蜱的防控。

关键词: 黑龙江省逊克县, 火山熔岩地区, 活蜱, 新发蜱传病原体, 复合感染

Abstract:

Objective To investigate ticks that harbor emerging tick-borne pathogens and co-infection in lava area of Xunke county of Heilongjiang. Methods A total of 257 live ticks were collected in April to June, 2015. All samples were amplified for specific fragments of 6 emerging tick-borne pathogens by PCR, and further identified through gene sequencing. Results The results showed that 86 positive samples of spotted fever group Rickettsia, infection prevalence was 33.46%; Five positive samples of Bartonella, infection prevalence was 1.94%; 11 positive samples of Anaplasma phagocytophilum, infection prevalence was 4.28%; DNA of Borrelia burgdorferi, Babesia, Ehrlichiosis detection results were all negative. It was also found that 8 co-infection samples existed in Dermacentor silvarum, Ixodes persuleatu, and Haemaphysalis concinna, and the infection prevalence was 3.11%. It was confirmed that co-infection of spotted fever group Rickettsia and Bartonella existed in D. silvarum and coinfection of spotted fever group Rickettsia and An. phagocytophilum existed in I. persuleatus and H. concinna. Conclusion To strengthen the prevention and control of vector ticks in lava area of Xunke county of Heilongjiang is warranted.

Key words: Xunke county of Heilongjiang, Lava area, Live ticks, Emerging tick-borne pathogens, Co-infection

中图分类号:

杨军, 王艳梅, 鞠文东, 焦丹, 徐宁, 梁慧杰, 王晓杰, 付维明, 耿聪, 程成. 逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染调查[J]. 中国媒介生物学及控制杂志, 2016, 27(4): 341-344.

YANG Jun, WANG Yan-mei, JU Wen-dong, JIAO Dan, XU Ning, LIANG Hui-jie, WANG Xiao-jie, FU Wei-ming, GENG Cong, CHENG Cheng. Investigation on emerging tick-borne pathogens and co-infection in ticks in lava area of Xunke county, Heilongjiang province[J]. Chines Journal of Vector Biology and Control, 2016, 27(4): 341-344.

参考文献

[1] 路景龙. 红星火山岩地质公园晋升省级公园占地1 685平方公里[EB/OL].(2008-11-24)[2016-02-01]. http://heilongjiang. dbw.cn/system/2008/11/24/051617106.shtml.
[2] 中华人民共和国国家质量监督检验检疫总局. SN/T 3392－2012 国境口岸莱姆病螺旋体检验规程[S]. 北京:中国标准出版社, 2013.
[3] Beelitz P, Schumacher S, Marholdt F, et al. Studies on the prevalence of Babesia canis canis in marsh ticks(Dermacentor reticulatus) in the Saarland [J]. Berl Munch Tierarztl Wochenschr, 2012, 125(3/4):168-171.
[4] Brouqui P, Bacellar F, Baranton G, et al. Guidelines for the diagnosis of tick - borne bacterial diseases in Europe[J]. Clin Microbiol Infect, 2004, 10(12):1108-1132.
[5] Otranto D, Dantas-Torres F, Giannelli A, et al. Ticks infesting humans in Italy and associated pathogens[J]. Parasit Vectors, 2014, 7:328.
[6] 宋锋林, 王静, 张晓龙, 等. 蜱类携带粒细胞无形体的核酸检测及核酸序列分析[J]. 中国国境卫生检疫杂志, 2011, 34 (5):348-350.
[7] 高东旗, 曹务春, 张习坦, 等. 野外采集标本中人埃立克体16S rRNA 基因扩增方法及应用[J]. 寄生虫与医学昆虫学报, 2001, 8(3):175-180.
[8] 杨军, 徐文兴, 白阳森, 等. 中国逊克和俄罗斯波亚尔科沃边境蜱类监测分析[J]. 中华卫生杀虫药械, 2013, 19(4): 329-331.
[9] 鞠文东, 程成, 付维明, 等. 黑龙江口岸斑点热群立克次体调查研究[J]. 寄生虫与医学昆虫学报, 2015, 22(1):37-43.
[10] Jia N, Zheng YC, Jiang JF, et al. Human infection with Candidatus Rickettsia tarasevichiae[J]. N Engl J Med, 2013, 369 (12):1178-1180.
[11] Jia N, Zheng YC, Ma L, et al. Human infections with Rickettsia raoultii, China[J]. Emerg Infect Dis, 2014, 20(5):866-868.
[12] 杨军, 鞠文东, 焦丹, 等. 黑龙江省逊克县火山熔岩地区啮齿动物携带病原体调查[J]. 中国媒介生物学及控制杂志, 2015, 26(3):238-241.
[13] 黄海楠, 丁壮, 何静, 等. 部分省区啮齿动物中斑点热群立克次体的检测[J]. 中国媒介生物学及控制杂志, 2006, 17(2): 83-85.
[14] 左双燕, 唐琨, 郑元春, 等. 黑龙江林区野鼠中斑点热群立克次体的核酸检测与序列分析[J]. 中南大学学报:医学版, 2013, 38(5):443-447.
[15] 程成, 鞠文东, 付维明, 等. 黑龙江密山口岸3种蜱媒传染病在媒介蜱中复合感染的调查[J]. 中国公共卫生管理, 2015, 31 (3):361-363.
[16] Sun Y, Liu GP, Yang LW, et al. Multiple infections of tick- borne pathogens in Ixodes persulcatus collected from forests in Heilongjiang province[J]. Acta Parasitol Med Entomol Sin, 2007, 14(4):231-240.
[17] 唐琨, 左双燕, 李颖, 等. 黑龙江省旅游区蜱伯氏疏螺旋体和斑点热群立克次体复合感染的动态调查[J]. 中华流行病学杂志, 2012, 33(5):513-516.

逊克县火山熔岩地区蜱携带新发蜱传病原体及复合感染调查

Investigation on emerging tick-borne pathogens and co-infection in ticks in lava area of Xunke county, Heilongjiang province

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