宁夏不同栖息环境啮齿动物巴尔通体感染状况调查

doi:10.11853/j.issn.1003.8280.2016.03.006

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (3): 235-240.DOI: 10.11853/j.issn.1003.8280.2016.03.006

宁夏不同栖息环境啮齿动物巴尔通体感染状况调查

姜亚运^1,2, 鲁亮², 宋秀平², 岳玉娟², 王君², 刘起勇², 李锦春¹, 栗冬梅²

1 安徽农业大学动物科技学院, 合肥 230036;
2 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 感染性疾病诊治协同创新中心, 北京 102206

收稿日期:2016-03-02 出版日期:2016-06-20 发布日期:2016-06-20
通讯作者: 李锦春,Email:jinchunli64@163.com;刘起勇,Email:liuqiyong@icdc.cn
作者简介:姜亚运,男,在读硕士,主要从事巴尔通体病原学及分子生物学研究,Email:642300281@qq.com
基金资助:
国家科技重大专项课题(2012ZX10004-219)

Investigation of the Bartonella infection in wild rodent populations in Ningxia Hui Autonomous Region, China

JIANG Ya-yun^1,2, LU Liang², SONG Xiu-ping², YUE Yu-juan², WANG Jun², LIU Qi-yong², LI Jin-chun¹, LI Dong-mei²

1 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, Anhui Province, China;
2 State Key Laboratory of Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases

Received:2016-03-02 Online:2016-06-20 Published:2016-06-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2012ZX10004-219)

摘要/Abstract

摘要：

目的调查宁夏地区啮齿动物巴尔通体感染状况,了解当地鼠传巴尔通体物种组成。方法用夹夜法在宁夏不同地区、不同生境捕获啮齿动物,无菌操作取肝和脾,应用PCR扩增巴尔通体特异性gltA基因鉴定菌株,对阳性标本进一步扩增,ftsZ、rpoB和16S rRNA基因测序并进行序列分析。用Mega 6.06 软件进行比对,应用邻接法构建系统发育树。结果从贺兰山、银川市、中卫市和固原市选择14个采样点,涵盖草原、灌丛、农田和林地4种生境,共采集啮齿动物样品234份,分离培养巴尔通体51株,感染率为21.79% (51/234)。共捕获啮齿动物13属20种,有7属9种啮齿动物分离出巴尔通体。阿拉善黄鼠、黑线姬鼠、大林姬鼠、三趾跳鼠、子午沙鼠、长尾仓鼠、长爪沙鼠、洮州绒鼠和五趾跳鼠的感染率分别为45.45%(5/11)、33.33%(15/45)、33.33%(11/33)、25.00%(5/20)、20.83%(5/24)、17.86%(5/28)、14.29%(1/7)、13.64%(3/22) 和11.11%(1/9),阿拉善黄鼠感染率最高。系统发育分析提示分离的巴尔通体与格拉汉姆巴尔通体(Bartonella grahamii)、伊丽莎白巴尔通体(B. elizabethae)、非洲跳鼠巴尔通体(B. jaculi)、日本巴尔通体(B. japonica)和泰勒巴尔通体(B. taylorii)相似度较高。结论宁夏地区啮齿动物中存在巴尔通体自然感染,在多种鼠类、不同生境中均有分布,且感染率较高;序列分析显示巴尔通体基因型呈多样性,且具有致病菌种,人群暴露在相应环境中与鼠群接触存在感染致病的风险。

关键词: 巴尔通体, 啮齿动物, 系统发育分析

Abstract:

Objective To investigate the Bartonella infections in wild rodent populations in Ningxia Hui Autonomous Region, China, and provide support for the prevention and control of Bartonella infections in humans. Methods Rodents were collected from different regions and habitats by the night trapping method. The livers and spleens were sampled using aseptic technique and the specific primers of Bartonella spp. were used to amplify the gltA gene fragment using PCR. Then the ftsZ, rpoB, and 16S rRNA genes of the positive samples were amplified and positive amplicons were sequenced. The nucleic acid sequences were submitted to the GenBank. The multiple sequence alignment of the sequences was Mega 6.06 software. The neighbor-joining tree was inferred from gltA similarity data with Mega 6.06 software. Results Five sampling sites were selected from Helan Mountain, Yinchuan, and Zhongwei city as well as Guyuan. The sampling sites were categorized into four habitats including grassland, shrub, farmland and forest, from which 233 samples were collected. Bartonella was cultivated in 51 samples giving a positive rate of 21.79% (51/234). A total of 13 genera, 20 species of rodents were captured. The Bartonella was isolated from 9 species, 7 genera of rodents. The rates of isolation among different species were: 45.45% (5/11) in Spermophilus alaschanicus, 33.33% (15/45) in Apodemus agrarius, 33.33%(11/33) in Ap. peninsulae, 25.00% (5/20) in Dipus sagitta, 20.83% (5/24) in Meriones meridianus, 17.86% (5/28) in Cricetulus longicaudatus, 14.29% (1/7) in Meriones unguiculatus, 13.64% (3/22) in Cricetidae and 11.11% (1/9) in Allactaga sibirica. Spermophilus alaschanicus infection rate was highest. The Bartonella isolates were found to be most similar to B. grahamii, B. elizabethae, B. jaculi, B. japonica and B. taylorii. Conclusion This report provides evidence of Bartonella infections in wild rodent populations in Ningxia region. The high prevalence of Bartonella spp. exists in various rodents and habitats. Phylogenetic analysis showed high diversity in the Bartonella isolates comprising of the pathogenic species which could pose risk of human infections.

Key words: Bartonella, Rodents, Phylogenetic analysis

中图分类号:

S443
Q939.93

姜亚运, 鲁亮, 宋秀平, 岳玉娟, 王君, 刘起勇, 李锦春, 栗冬梅. 宁夏不同栖息环境啮齿动物巴尔通体感染状况调查[J]. 中国媒介生物学及控制杂志, 2016, 27(3): 235-240.

JIANG Ya-yun, LU Liang, SONG Xiu-ping, YUE Yu-juan, WANG Jun, LIU Qi-yong, LI Jin-chun, LI Dong-mei. Investigation of the Bartonella infection in wild rodent populations in Ningxia Hui Autonomous Region, China[J]. Chines Journal of Vector Biology and Control, 2016, 27(3): 235-240.

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宁夏不同栖息环境啮齿动物巴尔通体感染状况调查

Investigation of the Bartonella infection in wild rodent populations in Ningxia Hui Autonomous Region, China

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