高分辨率熔解曲线分析技术在物种鉴定与分型中的应用

doi:10.11853/j.issn.1003.8280.2016.02.030

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (2): 206-208.DOI: 10.11853/j.issn.1003.8280.2016.02.030

高分辨率熔解曲线分析技术在物种鉴定与分型中的应用

何江¹, 易海华², 周鹏程³, 崔北金¹, 吴炳耀³, 孙立新³

1 安徽理工大学医学院, 安徽淮南 232001;
2 徐州出入境检验检疫局, 江苏徐州 221000;
3 江苏出入境检验检疫局, 南京 210001

收稿日期:2015-12-23 出版日期:2016-04-20 发布日期:2016-04-20
通讯作者: 孙立新,Email:slxdt63@163.com
作者简介:何江,男,在读硕士,主要从事分子生物学研究工作,Email:hejaust@163.com
基金资助:
江苏出入境检验检疫局科研基金项目(2014KJ41,2016KJ55)

Application of high resolution melting curve analysis technology on the species identification and classification

HE Jiang¹, YI Hai-hua², ZHOU Peng-cheng³, CUI Bei-jin¹, WU Bing-yao³, SUN Li-xin³

1 School of Medicine, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China;
2 Xuzhou Entry-Exit Inspection and Quarantine Bureau;
3 Jiangsu Entry-Exit Inspection and Quarantine Bureau

Received:2015-12-23 Online:2016-04-20 Published:2016-04-20
Supported by:
Supported by the Science and Technology Project of the Jiangsu Entry-Exit Inspection and Quarantine Bureau (No. 2014KJ41, 2016KJ55)

摘要/Abstract

摘要：

高分辨率熔解曲线分析技术是在熔解曲线分析基础上,利用饱和染料替代非饱和染料,借助专业高分辨率仪器,应用于基因突变、单核苷酸多态性和基因分型的新方法。具有灵敏度高、特异性强、避免污染、操作简便、速度快、通量高等优点,在昆虫、细菌、真菌、原虫鉴定及病毒分型等相关领域应用广泛。

关键词: 高分辨率熔解曲线, 物种鉴定, 分型

Abstract:

High resolution melting (HRM) curve analysis technology is a new method applied to genotyping and detection of gene mutations and single nucleotide polymorphism. The new method on the basis of the melting curve analysis, replaces the unsaturated dye with the saturation one, and with the aid of the professional high resolution instruments. HRM has received much concerns in the related fields for its advantages, such as high sensitivity and specificity, contamination avoidance, ease of operation, higher speed and flux, it has been widely used in the identification of insects, bacteria, fungi, protozoa and the classification of virus.

Key words: High resolution melting, Species identification, Classification

中图分类号:

Q754

何江, 易海华, 周鹏程, 崔北金, 吴炳耀, 孙立新. 高分辨率熔解曲线分析技术在物种鉴定与分型中的应用[J]. 中国媒介生物学及控制杂志, 2016, 27(2): 206-208.

HE Jiang, YI Hai-hua, ZHOU Peng-cheng, CUI Bei-jin, WU Bing-yao, SUN Li-xin. Application of high resolution melting curve analysis technology on the species identification and classification[J]. Chines Journal of Vector Biology and Control, 2016, 27(2): 206-208.

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高分辨率熔解曲线分析技术在物种鉴定与分型中的应用

Application of high resolution melting curve analysis technology on the species identification and classification

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