多位点序列分型技术在沃尔巴克氏体基因分型中的研究进展

doi:10.11853/j.issn.1003.8280.2016.02.028

中国媒介生物学及控制杂志 ›› 2016, Vol. 27 ›› Issue (2): 197-201.DOI: 10.11853/j.issn.1003.8280.2016.02.028

多位点序列分型技术在沃尔巴克氏体基因分型中的研究进展

胡亚萍^1,2, 刘小波², 桑少伟², 陈斌¹, 刘起勇²

1 重庆师范大学生命科学学院, 昆虫与分子生物学研究所, 重庆 401331;
2 中国疾病预防控制中心传染病预防控制所, 世界卫生组织媒介生物监测与管理合作中心, 传染病预防控制国家重点实验室, 北京 102206

收稿日期:2015-10-28 出版日期:2016-04-20 发布日期:2016-04-20
通讯作者: 刘起勇,Email:liuqiyong@icdc.cn;陈斌,Email:c_bin@hotmail.com
作者简介:胡亚萍,在读硕士,主要从事蚊媒生物防控方面的研究,Email:huyap9009@163.com
基金资助:
国家科技重大专项课题(2012ZX10004219-002);国家自然科学基金(81273139)

Application of multilocus sequence typing on Wolbachia genotyping

HU Ya-ping^1,2, LIU Xiao-bo², SANG Shao-wei², CHEN Bin¹, LIU Qi-yong²

1 Institute of Entomology and Molecular Biology, College of Life Sciences, Chongqing Normal University, Chongqing 401331, China;
2 WHO Collaborating Centre for Vector Surveillance and Management, State Key Laboratory of Infectious iseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention

Received:2015-10-28 Online:2016-04-20 Published:2016-04-20
Supported by:
Supported by the National Science and Technology Major Project of China (No. 2012ZX10004219-002) and the National Natural Science Foundation of China (No. 81273139)

摘要/Abstract

摘要：

沃尔巴克氏体是一种广泛分布于节肢动物及线虫体内的胞内共生菌,能通过多种方式参与宿主生殖调控。多位点序列分型技术(MLST)是一种基于核酸序列测定的细菌分型方法,该方法通过PCR技术扩增多个管家基因内部片段并测定其序列,进而分析菌株变异。该文对沃尔巴克氏体分型及系统发育分析研究现状以及重点对MLST方法在沃尔巴克氏体中的研究进展进行综述,并对MLST技术在蚊媒生物防治中的应用进行展望。

关键词: 沃尔巴克氏体, 多位点序列分型, 基因分型

Abstract:

Wolbachia is a genus of endosymbiotic alphaproteo bacteria infecting a wide range of arthropods and filarial nematodes, which can manipulates the reproductive mode of its hosts in various kinds of ways. Multilocus sequence typing (MLST) was proposed as a nucleotide sequence-based approach that could be applied to many bacteria. It analyzed allelic variation at multiple housekeeping loci by nucleotide sequencing of internal fragments. Here we make an overview on the state of Wolbachia genotypes and phylogeny, and the development of MLST analysis on Wolbachia. We also make a prospect on the application of MLST in the biological control of mosquito-borne diseases.

Key words: Wolbachia, Multilocus sequence typing, Genotyping

中图分类号:

胡亚萍, 刘小波, 桑少伟, 陈斌, 刘起勇. 多位点序列分型技术在沃尔巴克氏体基因分型中的研究进展[J]. 中国媒介生物学及控制杂志, 2016, 27(2): 197-201.

HU Ya-ping, LIU Xiao-bo, SANG Shao-wei, CHEN Bin, LIU Qi-yong. Application of multilocus sequence typing on Wolbachia genotyping[J]. Chines Journal of Vector Biology and Control, 2016, 27(2): 197-201.

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多位点序列分型技术在沃尔巴克氏体基因分型中的研究进展

Application of multilocus sequence typing on Wolbachia genotyping

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