中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (6): 620-621.DOI: 10.11853/j.issn.1003.4692.2015.06.021

• 调查研究 • 上一篇    下一篇

青海省人间布鲁氏菌的分离培养和生物学特性

王建玲, 胡桂英, 马丽, 徐立青, 田广, 马俊英, 杨旭欣, 秦豫民, 赵延梅, 薛红梅, 金星, 杨宁海, 刘祖义   

  1. 青海省地方病预防控制所布病预防控制科, 西宁 811602
  • 收稿日期:2015-07-31 出版日期:2015-12-20 发布日期:2015-12-20
  • 通讯作者: 徐立青
  • 作者简介:王建玲,女,检验技师,主要从事布病防治检验工作,Email:m13086262342-1@163.com
  • 基金资助:

    国家自然科学基金(81160355);青海省科技计划项目(2013-Z749)

Isolation and biological characteristics of human brucellosis in Qinghai

WANG Jian-ling, HU Gui-ying, MA Li, XU Li-qing, TIAN Guang, MA Jun-ying, YANG Xu-xin, QIN Yu-min, ZHAO Yan-mei, XUE Hong-mei, JIN Xing, YANG Ning-hai, LIU Zu-yi   

  1. Qinghai Institute for Endemic Disease Prevention and Control, Xining 811602, Qinghai Province, China
  • Received:2015-07-31 Online:2015-12-20 Published:2015-12-20
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 81160355)and the Science and Technology Plan of Qinghai Province(No. 2013-Z749)

摘要:

目的 对青海省1958-2013年从人体分离到的布鲁氏菌进行分析。方法 对1958年以来,临床诊断为布鲁氏菌病(布病)患者分离出的43株布鲁氏菌的来源、菌培养与临床关系等进行整理分析,硫堇和碱性复红的染料抑菌试验、单项血清凝集反应、噬菌体裂解试验对43株布鲁氏菌进行菌株表型分型鉴定。结果 取材337份做布鲁氏菌培养,分离出43株,其中慢性患者15例局部病灶检出布鲁氏菌。43株均为羊种布鲁氏菌,其中3株为羊1型,14株为羊2型,26株为羊3型。结论 羊种菌是青海省人群布病的致病菌。

关键词: 布鲁氏菌, 临床关系

Abstract:

Objective Isolated Brucella from human during 1958 to 2013 in Qinghai was analyzed. Methods Since 1958, the clinical diagnosis of brucellosis patients in bacteria from 43 strains of Brucella isolated culture were source and analyzed with clinical relationship, using the hydrogen sulfide produced determination, thionine and basic fuchsin dye bacteriostasis test, single serum agglutination reaction, phage splitting test on 43 strains of Brucella strains were phenotype subtype identification. Results From 337 for Brucella culture, isolated 43 strains of Brucella. The 15 patients with localized lesions were detected in chronic brucellosis. The 43 strains were 3 strains of Brucella melitensis, sheep, sheep 1 type 14 strains of type 2, 26 type 3 strains of sheep. Conclusion Sheep bacteria are pathogenic bacteria of brucellosis in Qinghai population.

Key words: Brucella, Clinical relationship

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