长春市鼠类携带汉坦病毒的基因型分析

doi:10.11853/j.issn.1003.4692.2015.06.016

摘要/Abstract

摘要：

目的了解长春市鼠类动物所携带的汉坦病毒基因型别。方法采集长春市地区鼠肺样品,应用免疫荧光法检测鼠肺中汉坦病毒抗原,提取阳性样本中病毒RNA,利用RT-PCR法扩增S基因片段,并测序,利用DNAman软件和Mega 6.0 软件进行序列分析。结果在长春地区共捕获褐家鼠100 只,检测到阳性样本3 份,阳性率为3.0%。通过测序获得3株病毒的S基因片段序列,经同源性分析比较发现各序列间核苷酸同源性为100%,与其他汉城病毒(SEOV)核苷酸同源性在95.4%～100%之间,3株病毒全部为SEOV。系统发生树分析表明,3株病毒在同一组内,属于S3亚型。结论研究表明长春市褐家鼠中携带的汉坦病毒为SEOV型,S3亚型。

关键词: 汉坦病毒, 反转录-聚合酶链反应, 基因分型, 汉城病毒

Abstract:

Objective To study the genotypes of the Hantavirus carried by the rodents in Changchun city. Methods Rat lung samples were collected in Changchun areas, and Hantavirus antigens in rat lungs were detected by immunofluorescence assay. The S gene fragment in HV-positive samples were amplified by RT-PCR and sequenced. The gene sequences were analyzed by using DNAman software and Mega 6.0 software. Results In this study, 100 Rattus norvegicus were captured in Changchun areas, of which 3 (3.0%) samples were positive for HV antigen. The S gene fragment of 3 strains were amplified from 3 positive samples. Homology analysis indicated that the nucleotide homology from the three S segment sequences was 100%, while that to other SEOV nucleotide homology was 95.4%-100%. All the 3 strains were SEOV. Phylogenesis analysis showed that the 3 strains were in the same group on the phylogenetic tree, which belonged to S3 sub-genotype. Conclusion The study shows that the Hantavirus carried by R. norvegicus in Changchun city was SEOV, the sub-genotype SEOV-3.

Key words: Hantavirus, Reverse transcription-polymerase chain reaction, Genotype, Seoul virus

中图分类号:

R373.3

孙炳欣, 孙宇, 沈博, 吴静. 长春市鼠类携带汉坦病毒的基因型分析[J]. 中国媒介生物学及控制杂志, 2015, 26(6): 600-604.

SUN Bing-xin, SUN Yu, SHEN Bo, WU Jing. Genotype analysis of Hantavirus carried by the rodents in Changchun city[J]. Chines Journal of Vector Biology and Control, 2015, 26(6): 600-604.

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