山东省淡色库蚊核糖体DNA第二内转录间隔区基因多态性分析

doi:10.11853/j.issn.1003.4692.2015.06.003

中国媒介生物学及控制杂志 ›› 2015, Vol. 26 ›› Issue (6): 550-554.DOI: 10.11853/j.issn.1003.4692.2015.06.003

山东省淡色库蚊核糖体DNA第二内转录间隔区基因多态性分析

刘飞^1,2,3, 郭秀霞¹, 程鹏¹, 王海防¹, 刘丽娟¹, 杨培培¹, 赵玉强¹, 王怀位¹, 公茂庆¹

1 山东省医学科学院, 山东省寄生虫病防治研究所, 山东省医药卫生媒介生物学重点实验室, 山东济宁 272033;
2 济南大学山东省医学科学院医学与生命科学学院, 济南 250012;
3 济宁市第一人民医院, 山东济宁 272033

收稿日期:2015-08-21 出版日期:2015-12-20 发布日期:2015-12-20
通讯作者: 公茂庆
作者简介:刘飞,女,在读硕士,检验技师,从事病原生物学研究,Email:707137516@qq.com
基金资助:
国家自然科学基金(81271877,81471985)

Genetic polymorphism analysis on rNDA-ITS2 from different geographical strains of Culex pipiens pallens in Shandong province

LIU Fei^1,2,3, GUO Xiu-xia¹, CHENG Peng¹, WANG Hai-fang¹, LIU Li-juan¹, YANG Pei-pei¹, ZHAO Yu-qiang¹, WANG Huai-wei¹, GONG Mao-qing¹

1 Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Department of Medical Entomology, Shandong Province Key Laboratory of Health and Vector Biology, Jining 272033, Shandong Province, China;
2 School of Medicine and Life Sciences, University of Jinan, Shandong Academy of Medical Sciences, Jinan 250012, Shandong Province, China;
3 Shandong Jining No. 1 People's Hospital, Jining 272033, Shandong Province, China

Received:2015-08-21 Online:2015-12-20 Published:2015-12-20
Supported by:
Supported by the National Natural Science Foundation of China(No. 81271877, 81471985)

摘要/Abstract

摘要：

目的探讨山东省不同地区淡色库蚊核糖体DNA第二内转录间隔区(rDNA-ITS2)基因序列特征,分析其遗传变异。方法在山东省的济南、济宁、青岛、聊城、临沂、淄博市以及江苏省南京市采集淡色库蚊成蚊,并取实验室选育的抗氯氰菊酯与抗敌敌畏品系成蚊,提取蚊虫DNA,PCR扩增淡色库蚊的rDNA-ITS2,PCR产物纯化后连接至pMD19-T载体进行基因测序,生物信息学分析rDNA-ITS2 基因多态性。结果 PCR特异扩增山东省6个不同地理株及南京市和实验室2个不同品系的淡色库蚊rDNA-ITS2区域,获得460 bp左右的扩增片段。测序结果显示淡色库蚊rDNA-ITS2出现36个变异位点,表现出基因的多态性,其rDNA-ITS2基因单核苷酸存在转换、颠换和缺失。结论山东省不同地理区域和不同抗性的淡色库蚊在rDNA-ITS2区出现遗传多态性,可能与蚊虫生态环境有一定的关系。

关键词: 淡色库蚊, 核糖体DNA第二内转录间隔区, 基因多态性

Abstract:

Objective To explore gene sequence characteristics of rNDA-ITS2 in different populations of Culex pipiens pallens from Shandong province and analyze the genetic variation of these mosquitoes. Methods Adult-female mosquitoes were collected from different cities: Jinan, Jining, Qingdao, Liaocheng, Linyi, Zibo from Shandong and a sample from Nanjing. The pyrethroid-resistant and dichlorvos-resistant strains were reared in the laboratory. Single mosquito genomic DNA was extracted from Cx. pipiens pallens of these different populations and rNDA-ITS2 of Cx. pipiens pallens were specifically amplified by PCR. The PCR products were cloned into the pMD-19T simple vectors and sequenced. Genetic polymorphisms of them were analyzed by biological information systems. Results The obtained rDNA-ITS2 sequences were approximate 460 bp in length, with 36 mutation sites. Culex pipiens pallens from different geographical environment showed gene polymorphisms in sequence analysis of rNDA-ITS2. There were transition,transversion and deletion in the mono nucleotide of rNDA-ITS2 gene. Conclusion Genetic polymorphisms presented on rNDA-ITS2 of Cx. pipiens pallens from different geographical regions in Shandong province and different resistance strains in the lab were probably associated with the ecological environment.

Key words: Culex pipiens pallens, rDNA-ITS2, Genetic polymorphism

中图分类号:

R384.1

刘飞, 郭秀霞, 程鹏, 王海防, 刘丽娟, 杨培培, 赵玉强, 王怀位, 公茂庆. 山东省淡色库蚊核糖体DNA第二内转录间隔区基因多态性分析[J]. 中国媒介生物学及控制杂志, 2015, 26(6): 550-554.

LIU Fei, GUO Xiu-xia, CHENG Peng, WANG Hai-fang, LIU Li-juan, YANG Pei-pei, ZHAO Yu-qiang, WANG Huai-wei, GONG Mao-qing. Genetic polymorphism analysis on rNDA-ITS2 from different geographical strains of Culex pipiens pallens in Shandong province[J]. Chines Journal of Vector Biology and Control, 2015, 26(6): 550-554.

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山东省淡色库蚊核糖体DNA第二内转录间隔区基因多态性分析

Genetic polymorphism analysis on rNDA-ITS2 from different geographical strains of Culex pipiens pallens in Shandong province

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