关键词: 家蝇, 抗药性, 拟除虫菊酯, 细胞色素P450, 钠离子通道, 等位基因频率

Abstract:

Objective The housefly Musca domestica (Diptera: Muscidae), possess significant health concerns for humans and animals. Research on genetics and molecular biology of this pest is helpful for developing an effective control strategy. Methods Frequencies of pyrethroid resistance alleles were compared for five field-derived housefly populations collected across China over a three-years interval. Results Under the laboratory conditions without insecticide selection over almost 3 years, the frequencies of resistant allele (CYP6D1v1) increased in Guangdong, Shanghai, and Beijing populations, but declined in Shandong and Jilin populations that had low initial frequencies of CYP6D1v1 allele. Guangdong, Shandong and Jilin populations experienced a significant decline in the frequency of voltage-sensitive sodium channel resistance allele (kdr-his). The most notable change was observed in Shandong population, in which kdr-his disappeared from the initial frequency of 0.33. From these observations, we concluded that CYP6D1v1 may have fitness advantages and kdr-his allele has haplotype-variable fitness. Conclusion These findings reconfirm our previous suggestion that resistance management strategy should be customized for a given location.

Key words: Musca domestica, Insecticide resistance, Pyrethroid CYP6D1, Voltage-sensitive sodium channel, Allele frequency