DNA条形码技术在福建省蝇类鉴定中的应用

doi:10.11853/j.issn.1003.4692.2014.06.007

中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (6): 514-517.DOI: 10.11853/j.issn.1003.4692.2014.06.007

DNA条形码技术在福建省蝇类鉴定中的应用

吴荣泉^{1 ,2},张建庆³,方义亮³,肖武³,房长天³,刘宝英¹

1 泉州出入境检验检疫局综合技术服务中心,福建泉州 362000; 2 福建医科大学公共卫生学院,福州 350108; 3 福建国际旅行卫生保健中心

收稿日期:2014-04-26 出版日期:2014-12-20 发布日期:2014-12-20
通讯作者: 刘宝英,Email: liuby5@126.com
作者简介:吴荣泉,男,硕士,医师,主要从事虫媒传染病相关研究
基金资助:
国家质检总局科技计划项目(2013IK094); 福建出入境检验检疫局科技计划项目(FK2012-12)

Application of DNA barcoding in identification of fliesin Fujian province, China

WU Rong-quan^{1 ,2}, ZHANG Jian-qing³, FANG Yi-liang³, XIAO Wu³, FANG Chang-tian³, LIU Bao-ying²

1 Quanzhou Entry-Exit Inspection and Quarantine Bureau Comprehensive Technical Service Center, Quanzhou 362000, Fujian Province, China; 2 Public Health School of Fujian Medical University, Fuzhou 350108, Fujian Province, China;3 Fujian International Travel Healthcare Center

Received:2014-04-26 Online:2014-12-20 Published:2014-12-20
Contact: LIUBao-ying,Email:liuby5@126.com
Supported by:
Supported by the Science and Technology Plan Project of General Administration of Quality Supervision, Inspection Quarantine(No. 2013IK094) and Science and Technology Plan Project of Fujian Entry?Exit Inspection and Quarantine Bureau (No. FK2012-12)

摘要/Abstract

摘要： 目的　探讨DNA条形码技术应用于福建省蝇类鉴定的可行性,并初步建立福建省蝇类的基因数据库。方法　采集自福建省部分地区的4科13属19种64个蝇类新鲜标本,提取基因组DNA进行PCR扩增,序列测序,并进行同源性对比、进化距离分析,构建系统进化树。结果　基因序列分析结果显示,上述蝇类mtDNA上细胞色素C氧化酶亚基Ⅰ基因序列的种内进化分歧均数在0～3.32%之间;除铜绿蝇与丝光绿蝇进化分歧均数为0.69%外,其余种间进化分歧均数在5.26%～18.30%之间;系统进化树分析显示,相同种能够很好聚集在一起,Bootstrap 检验可信度均达96%以上。结论　DNA条形码技术可用于福建省一些常见蝇类的鉴定,作为形态学鉴定的补充手段。

关键词: 细胞色素C氧化酶亚基Ⅰ基因, 系统发育树, DNA条形码, 线粒体DNA

Abstract: Objective　To evaluate the feasibility of DNA barcoding applied to the identification of flies in Fujian province, China, and to set up the nucleic acid sequence database for flies in Fujian. Methods　Sixty-four fly specimens belonging to 19 species, 13 genera, and 4 families were collected from Fujian. Genomic DNA was extracted from the flies. Mitochondrial COⅠ gene was amplified by consensus primer PCR and sequenced. Sequence alignment and evolutionary divergence were performed, and a neighbor-joining phylogenetic tree was constructed based on these sequences. Results　Intraspecific divergence ranged between 0-3.32%; interspecific divergence ranged between 5.26%-18.30%, except for Lucilia cuprina and Lucilia sericata, which had only 0.69% divergence from each other. The result of phylogenetic tree showed that sequences from the same species gathered closely with each other. The reliability of Bootstrap test was all above 96%. Conclusion　DNA barcoding can be effectively used to identify some flies in Fujian province. It can be used as an efficient method supplementary to morphological identification.

Key words: COⅠ gene, Phylogenetic tree, DNA barcoding, mtDNA

中图分类号:

R384.2

吴荣泉,张建庆,方义亮,肖武,房长天,刘宝英. DNA条形码技术在福建省蝇类鉴定中的应用[J]. 中国媒介生物学及控制杂志, 2014, 25(6): 514-517.

WU Rong-quan, ZHANG Jian-qing, FANG Yi-liang, XIAO Wu, FANG Chang-tian, LIU Bao-ying. Application of DNA barcoding in identification of fliesin Fujian province, China[J]. Chines Journal of Vector Biology and Control, 2014, 25(6): 514-517.

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DNA条形码技术在福建省蝇类鉴定中的应用

Application of DNA barcoding in identification of fliesin Fujian province, China

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