关键词: 肾综合征出血热, 汉坦病毒, 单核苷酸多态性

Abstract: Objective　To analyze the single nucleotide polymorphisms (SNPs) in HLA-B gene and HLA-DQ gene in hemorrhagic fever with renal syndrome (HFRS) patients and healthy people and to study the relationship between Hantavirus infection and genetic characteristics of humans. Methods　Five SNPs in HLA-B gene and HLA-DQ gene were amplified using allele-specific primer PCR (ASP-PCR). The association between SNPs and Hantavirus infection was investigated by case-control study. Results　According to the result of the HFRS antibody diagnostic assay, 24 Hantavirus-positive whole blood samples were included. Negative samples were collected from 51 healthy people. The frequencies of CC genotype of rs34933313 in the case group and control group were 4.17% and 29.41%, respectively; the frequencies of GG genotype were 12.50% and 15.69%, respectively; the frequencies of CG genotype were 83.33% and 54.90%, respectively. The frequencies of CC and GG in the case group were significantly lower than those in the control group, and the frequency of CG genotype in the case group was significantly higher than that in the control group; significant difference in the genotype of rs34933313 was found between the two groups (χ2＝7.050, P＝0.029). No significant difference was found at any of the other four sites. Conclusion　SNP at rs34933313 might be associated with Hantavirus infection and may increase the risk of Hantavirus infection in humans. HLA-B gene is probably related to Hantavirus infection in humans.

Key words: Hemorrhagic fever with renal syndrome, Hantavirus, Single nucleotide polymorphism