关键词: 布鲁氏菌病诊断, 虎红平板凝集试验, 试管凝集试验, 截断值

Abstract: Objective To explore the feasibility of dilution rose bengal plate agglutination test (RBPT) for the diagnosis of brucellosis.Methods Serum samples from 93 brucellosis patients who came from Inner Mongolia, China in 2013 were tested by both dilution RBPT and standard tube agglutination test (SAT). According to the correlation between the results of the two tests and using the results of SAT as criteria, the sensitivity and specificity of RBPT were analyzed at different dilutions as cut-off values to evaluate the diagnostic accuracy.Results The area under the receiver operating characteristic curve for dilution RBPT was 0.946. When taking1∶2 as the cut-off value, the Youden index was 0.893, the sensitivity was 89.30% , and the specificity was 100%; when taking1∶4 as the cut-off value, the Youden index was 0.631, the sensitivity was 63.10%, and the specificity was 100%; when taking1∶8 as the cut-off value, the Youden index was 0.571, the sensitivity was 57.14%, and the specificity was 100%; when taking1∶16 as the cut-off value, the Youden index was 0.191, the sensitivity was 19.05%, and the specificity was 100%.Conclusion Dilution RBPT has the highest diagnostic accuracy at a dilution of1∶2, which provides a reference for the diagnosis of brucellosis.

Key words: Diagnosis of brucellosis, Rose bengal plate agglutination test, Tube agglutination test, Cut-offvalue