白蚁内源性纤维素酶基因的异源表达

doi:10.11853/j.issn.1003.4692.2014.03.024

摘要/Abstract

摘要：

作为含量最多的生物质, 纤维素是地球上储量最大的可再生资源。酶解纤维素是高效转化木质纤维素类生物质生产生物乙醇的重要途径。食木性白蚁是纤维素消化率最高的昆虫, 其唾液腺组织和中肠细胞都能分泌纤维素酶。该文从白蚁的内源性纤维素酶——内切-β-1, 4-葡聚糖酶(EG)和β-葡萄糖苷酶(BG)的分泌情况着手, 综述了其编码基因EG(GHF9)和BG(GHF1)的异源表达研究进展, 为进一步挖掘和研究白蚁体内丰富的纤维素酶基因提供了有益的背景信息和思路。

关键词: 白蚁, 内源性纤维素酶, EG(GHF9), BG(GHF1), 异源表达

Abstract:

As the primary component of biomass, cellulase is the richest renewable resource on the earth. Enzymatic hydrolysis of cellulose plays an important role in producing bioethanol by efficient conversion of lignocellulosic biomass. Termites, living on lignocellulose, possess the highest digestive efficiency for cellulose. Cellulases are secreted from termites’ salivary glands and midgut. This paper describes the secretion of termites’ endogenous cellulases, endo-β-1, 4-glucanase (EG) and β-glucosidase (BG), and reviews the research advances in heterologous expression of the corresponding encoding genes, EG (GHF9) and BG (GHF1). This will provide beneficial information and new insights into further discovery and study on the rich cellulase genes in termites.

Key words: Termite, Endogenous cellulase, EG(GHF9), BG(GHF1), Heterologous expression

中图分类号:

Q966

章珍, 莫建初. 白蚁内源性纤维素酶基因的异源表达[J]. 中国媒介生物学及控制杂志, 2014, 25(3): 281-283.

ZHANG Zhen, MO Jian-chu. Heterologous expression of termites’ endogenous cellulase genes[J]. Chines Journal of Vector Biology and Control, 2014, 25(3): 281-283.

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