滞育及冷暴露对白纹伊蚊卵Hsp70基因表达的影响

doi:10.11853/j.issn.1003.4692.2014.03.004

中国媒介生物学及控制杂志 ›› 2014, Vol. 25 ›› Issue (3): 211-214.DOI: 10.11853/j.issn.1003.4692.2014.03.004

滞育及冷暴露对白纹伊蚊卵Hsp70基因表达的影响

李玉伟, 鲁亮, 刘起勇

中国疾病预防控制中心传染病预防控制所媒介生物控制室, 世界卫生组织媒介生物监测与管理合作中心, 传染病预防控制国家重点实验室, 北京 102206

收稿日期:2014-03-07 出版日期:2014-06-20 发布日期:2014-06-20
通讯作者: 鲁亮, Email: luliang@icdc.cn
作者简介:李玉伟, 男, 在读硕士, 主要从事媒介生物病原生物学及相关公共卫生研究。
基金资助:
国家重大科学研究计划(973项目)(2012CB955501)

Effects of diapause and cold exposure on expression of Hsp70in Aedes albopictus eggs

LI Yu-wei, LU Liang, LIU Qi-yong

WHO Collaborating Centre for Vector Surveillance and Management, State Key Laboratory for Infectious Disease Preventionand Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

Received:2014-03-07 Online:2014-06-20 Published:2014-06-20
Contact: LU Liang, Email: luliang@icdc.cn

摘要/Abstract

摘要： 目的研究白纹伊蚊卵在滞育以及冷暴露条件下, 热休克蛋白70基因(Hsp70)的表达情况, 探究Hsp70在增强白纹伊蚊卵耐寒性方面的作用。方法以无冷暴露的非滞育卵作为对照组, 采用RT?qPCR方法检测白纹伊蚊滞育卵和非滞育卵Hsp70 mRNA的表达情况。结果室温25 ℃条件下, 滞育卵与非滞育卵表达的Hsp70差异无统计学意义(P＝0.392)。与对照组相比, 冷暴露后蚊卵Hsp70表达量均增加, 且滞育卵Hsp70的表达量明显高于非滞育卵的表达量, 分别是其对照组的16.44和5.22倍。结论 Hsp70并不是白纹伊蚊滞育期间的重要组成部分, 但是在冷暴露的恢复期表达量上调。

关键词: 白纹伊蚊, 卵, 冷暴露, 滞育, 热休克蛋白70基因

Abstract: Objective To investigate the expression of Hsp70 in Aedes albopictus diapausing and nondiapausing eggs after cold exposure and to determine the role of Hsp70 in enhancing cold tolerance of Ae. albopictus eggs. Methods Hsp70 mRNA expression in Ae. albopictus diapausing and nondiapausing eggs after cold exposure was determined by real?time quantitative PCR, using non?cold?exposed nondiapausing eggs as a control. Results No significant difference in Hsp70 mRNA expression was observed between diapausing and nondiapausing eggs at room temperature (25 ℃) (P＝0.392). Hsp70 mRNA expression increased in both diapausing and nondiapausing eggs after cold exposure, as compared with that of the control. Furthermore, the diapausing eggs had a higher amount of Hsp70 mRNA than the nondiapausing eggs; the expression levels in diapausing and nondiapausing eggs were 16.44 and 5.22 times, respectively, that of the control. Conclusion The expression of Hsp70 is usually not up?regulated during diapausing process, but it is up?regulated during recovery from cold exposure.

Key words: Aedes albopictus, Egg, Cold exposure, Diapause, Hsp70

中图分类号:

R384.1

李玉伟, 鲁亮, 刘起勇. 滞育及冷暴露对白纹伊蚊卵Hsp70基因表达的影响[J]. 中国媒介生物学及控制杂志, 2014, 25(3): 211-214.

LI Yu-wei, LU Liang, LIU Qi-yong. Effects of diapause and cold exposure on expression of Hsp70in Aedes albopictus eggs[J]. Chines Journal of Vector Biology and Control, 2014, 25(3): 211-214.

参考文献

[1] 吴家红, 程金芝, 陈璐, 等. 白纹伊蚊β?actin基因实时荧光定量RT?PCR方法的建立[J]. 四川动物, 2010, 29(5)：593-595.
[2] Mcdonald JR, Head J, Bale JS, et al. Cold tolerance, overwintering and establishment potential of Thrips palmi[J]. Physiol Entomol, 2000, 25(2)：159-166.
[3] Hanson SM, Cralg GB Jr. Aedes albopictus (Diptera： Culicidae) eggs： field survivorship during northern Indiana winters[J]. J Med Entomol, 1995, 32(5)：599-604.
[4] Reynolds JA, Poelchau MF, Rahman Z, et al. Transcript profiling reveals mechanisms for lipid conservation during diapause in the mosquito, Aedes albopictus[J]. J Insect Physiol, 2012, 58(7)： 966-973.
[5] Hanson SM, Cralg GB Jr. Cold acclimation, diapause, and geographic origin affect cold hardiness in eggs of Aedes albopictus (Diptera： Culicidae)[J]. J Med Entomol, 1994, 31(2)：192-201.
[6] 王海鸿, 雷仲仁. 昆虫热休克蛋白的研究进展[J]. 中国农业科学, 2005, 38(10)：2023-2034.
[7] Goto SG, Kimura MT. Heat- and cold?shock responses and temperature adaptations in subtropical and temperate species of Drosophila[J]. J Insect Physiol, 1998, 44(12)：1233-1239.
[8] Sinclair BJ, Gibbs AG, Roberts SP. Gene transcription during exposure to, and recovery from, cold and desiccation stress in Drosophila melanogaster[J]. Insect Mol Biol, 2007, 16(4)： 435-443.
[9] Jiang X, Zhai H, Wang L, et al. Cloning of the heat shock protein 90 and 70 genes from the beet armyworm, Spodoptera exigua, and expression characteristics in relation to thermal stress and development[J]. Cell Stress Chaperones, 2012, 17(1)：67-80.
[10] Kostal V, Tollarova?borovanska M. The 70 kDa heat shock protein assists during the repair of chilling injury in the insect, Pyrrhocoris apterus[J]. PLoS One, 2009, 4(2)：e4546.
[11] Denlinger DL, Rinehart JP, Yocum GD. Stress proteins： A role in insect diapause？[M]// Denlinger DL, Giebultowicz JM, Saunders DS. Insect Timing： Circadian Rhythmicity to Seasonality. Amsterdam：Elsevier Science B.V, 2001：155-171.
[12] Rinehart JP, Yocum GD, Denlinger DL. Developmental upregulation of inducible Hsp70 transcripts, but not the cognate form, during pupal diapause in the flesh fly, Ssarcophaga crassipalpis[J]. Insect Biochem Mol Biol, 2000, 30(6)：515-521.
[13] Hayward SA, PavlidesA SC, Tammariello SP, et al. Temporal expression patterns of diapause?associated genes in flesh fly pupae from the onset of diapause through post?diapause quiescence[J]. J Insect Physiol, 2005, 51(6)：631-640.
[14] 肖海军, 魏兆军, 薛芳森. 昆虫滞育关联热休克蛋白的研究进展 [J]. 昆虫学报, 2011, 54(9)：1068-1075.
[15] Rinehart JP, Li A, Yocum GD, et al. Up?regulation of heat shock proteins is essential for cold survival during insect diapause[J]. Proc Natl Acad Sci, 2007, 104(27)：11130-11137.
[16] 张永强, 王进军, 丁伟, 等. 昆虫热休克蛋白的研究概况[J]. 昆虫知识, 2004, 41(1)：16-19.
[17] Moribe Y, Oka K, Niimi T, et al. Expression of heat shock protein 70a mRNA in Bombyx mori diapause eggs[J]. J Insect Physiol, 2010, 56(9)：1246-1252.
[18] Yocum GD, Kemp WP, Bosch J, et al. Temporal variation in overwintering gene expression and respiration in the solitary bee Megachile rotundata[J]. J Insect Physiol, 2005, 51(6)：621-629.
[19] Yocum GD. Differential expression of two Hsp70 transcripts in response to cold shock, thermoperiod, and adult diapause in the Colorado potato beetle[J]. J Insect Physiol, 2001, 47(10)： 1139-1145.
[20] Tachibanas I, Numata H, Goto SG. Gene expression of heat?shock proteins (Hsp23, Hsp70 and Hsp90) during and after larval diapause in the blow fly Lucilia sericata[J]. J Insect Physiol, 2005, 51(6)：641-647.
[21] Goto SG, Yoshida KM, Kimura MT. Accumulation of Hsp70 mRNA under environmental stresses in diapausing and nondiapausing adults of Drosophila triauraria[J]. J Insect Physiol, 1998, 44(10)： 1009-1015.
[22] Goto SG, Kimura MT. Heat?shock?responsive genes are not involved in the adult diapause of Drosophila triauraria[J]. Gene, 2004, 326：117-122.
[23] Robich RM, Rinehart JP, Kitchenl J, et al. Diapause?specific gene expression in the northern house mosquito, Culex pipiens L., identified by suppressive subtractive hybridization[J]. J Insect Physiol, 2007, 53(3)：235-245.
[24] Rinehart JP, Robich RM, Denlinger DL. Enhanced cold and desiccation tolerance in diapausing adults of Culex pipiens, and a role for Hsp70 in response to cold shock but not as a component of the diapause program[J]. J Med Entomol, 2006, 43(4)：713-722.
[25] Zhang Q, Denlinger DL. Molecular characterization of heat shock protein 90, 70 and 70 cognate cDNAs and their expression patterns during thermal stress and pupal diapause in the corn earworm[J]. J Insect Physiol, 2010, 56(2)：138-150.

滞育及冷暴露对白纹伊蚊卵Hsp70基因表达的影响

Effects of diapause and cold exposure on expression of Hsp70in Aedes albopictus eggs

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	孙钦同, 韩英男, 刘言, 王学军, 刘峰, 赖世宏, 景晓. 山东省登革热媒介白纹伊蚊生态学调查分析[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 16-20.
[2]	李天奇, 刘钦梅, 吴瑜燕, 罗明宇, 王金娜, 龚震宇. 浙江省登革热重点地区2020年白纹伊蚊幼蚊种群密度监测分析[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 21-24.
[3]	魏超, 马海芳, 廖宁, 熊绮梦. 广西壮族自治区2020－2021年登革热媒介伊蚊密度专项调查结果分析[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 25-29.
[4]	赵春春, 周欣欣, 李文玉, 伦辛畅, 刘小波, 吴海霞, 王君, 刘起勇, 孟凤霞. 2020年中国13省份登革热媒介白纹伊蚊抗药性监测及分析研究[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 30-37.
[5]	郑宇婷, 杨春梅, 杨明东, 姜进勇. 云南边境地区登革热媒介伊蚊生态学及抗药性监测[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 38-43.
[6]	涂涛田, 肖汉森, 孟凤霞, 刘起勇, 刘小波, 何亚明, 季恒青. 重庆市2019年登革热暴发疫情应急控制后媒介伊蚊抗药性调查[J]. 中国媒介生物学及控制杂志, 2022, 33(1): 44-47.
[7]	周欣欣, 李芬, 段文波, 马欣然, 宋秀平, 吴少英, 孟凤霞. 白纹伊蚊电压门控钠离子通道基因克隆及其生物信息学分析[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 672-679.
[8]	薛海滨, 孟金霞, 任乐, 景晓. 山东省临沂市2018－2020年白纹伊蚊幼蚊监测与风险评估[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 686-690.
[9]	张育富, 张守刚, 朱丁, 顾灯安, 葛小伍, 袁建明, 崔倩, 褚宏亮. 江苏省多层住宅居民区不同楼层白纹伊蚊分布调查[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 700-704.
[10]	周毅彬, 朱奕奕, 朱伟, 姚隽一, 朱江, 冷培恩, 吴寰宇. 诱蚊诱卵器监测的空间抽样方法研究[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 749-755.
[11]	朱伟, 刘翔宇, 周毅彬. 绿篱喷洒控制白纹伊蚊密度的时空变化研究[J]. 中国媒介生物学及控制杂志, 2021, 32(6): 772-778.
[12]	郭秀霞, 程鹏, 刘丽娟, 王海防, 张崇星, 王怀位, 公茂庆. 白纹伊蚊紫外光敏感视蛋白基因的克隆及序列特征[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 553-559.
[13]	张家林, 黄发源. 安徽省2015－2019年登革热媒介伊蚊监测结果分析[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 582-585.
[14]	任飞林, 沈建勇, 付云, 刘光涛, 彭财伟. 布雷图指数与诱蚊诱卵指数监测白纹伊蚊幼蚊密度的重复测量分析[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 608-612.
[15]	邓惠, 刘礼平, 许齐爱, 廉战民, 段金花, 陈宗晶, 芦瑞鹏, 沈秀婷, 阴伟雄, 吴军, 林立丰. 3种蚊虫引诱剂与不同流量CO₂对白纹伊蚊诱集效果的研究[J]. 中国媒介生物学及控制杂志, 2021, 32(5): 637-641.