广东省流行的3株登革热2型病毒基因组全序列测定及分析

doi:10.11853/j.issn.1003.4692.2013.05.004

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (5): 392-396.DOI: 10.11853/j.issn.1003.4692.2013.05.004

广东省流行的3株登革热2型病毒基因组全序列测定及分析

白志军¹, 张培², 洪文艳², 刘建伟², 狄飚¹, 杨智聪¹, 任瑞文², 方美玉², 林立辉²

1 广州市疾病预防控制中心病毒免疫科, 广东广州 510080;
2 广州军区疾病预防控制中心, 广东广州 510507

收稿日期:2013-04-26 出版日期:2013-10-20 发布日期:2013-10-20
通讯作者: 任瑞文, Email: renruiwen@hotmail.com
作者简介:白志军(1977- ),男,硕士,副主任技师,主要从事病原学研究。Email: junewhite@163.com;张培(1979- ),女,硕士,助理研究员,主要从事病原学研究。Email: zhangpei7982@163.com
基金资助:
美国国立卫生研究院项目(1R01AI083202-01A1); 国家科技重大专项(2012ZX10004-213-005); 广东省科技计划项目(2011B031500011); 广州市科技计划项目(2010Y1-C151,2012Y2-00020); 广州市医药卫生科技项目(201102A212006, 20121A011122)

Determination and analysis of complete genomic sequences of three Dengue 2 virus strains isolated in Guangdong province, China

BAI Zhi-jun¹, ZHANG Pei², HONG Wen-yan², LIU Jian-wei², DI Biao¹, YANG Zhi-cong¹, REN Rui-wen², FANG Mei-yu², LIN Li-hui²

1 Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, Guangdong Province, China;
2 Center for Disease Control and Prevention of Guangzhou Military District, Guangzhou 510507, Guangdong Province, China

Received:2013-04-26 Online:2013-10-20 Published:2013-10-20
Supported by:
Supports by the National Institutes of Health Project (No. 1R01AI083202-01A1), National Science and Technology Major Project of China (No. 2012ZX10004-213-005), Guangdong Science and Technology Department Program (No. 2011B031500011), Guangzhou Science and Technology Department Program (No. 2010Y1-C151, 2012Y2-00020) and Science and Technology Program of Guangzhou Health Department (No. 201102A212006, 20121A011122)

摘要/Abstract

摘要：

目的测定广东省3株登革热2型病毒的全序列,探讨其来源及基因型。方法运用RT-PCR法扩增来自广东省的登革热2型GD09/93、GD05/98和GD19/2001病毒株的全序列,采用遗传距离法构建进化树。结果 3株登革热2型病毒的全基因组长度均为10 723 nt, 5'及3'端各有一非编码区,结构基因和非结构基因位于基因组97～10 269 nt之间,共编码3391个氨基酸,读码结构完全相同。GD05/98与GD09/93、GD05/98与GD19/2001、GD09/93与GD19/2001之间的碱基序列同源性分别为93.3%、92.4%和97.6%,氨基酸序列同源性分别为96.7%、96.5%和98.5%。结论 3株登革热2型病毒均对乳鼠致病,与对乳鼠不致病的参考株(DEN2-04株)比较共有18个氨基酸位点的差异引起极性或电荷变化,PrM-134、NS2A-153、NS4B-102所带电荷的变化对抗原性影响较大。GD05/98株与泰国分离株同为Ⅱ基因型,GD09/93和GD19/2001株与印度尼西亚、澳大利亚、台湾株分在Ⅳ基因型; 表明我国登革热2型病毒有不同的基因型,而不同时期也存在同一种基因型传播。

关键词: 登革热病毒, 全序列, 系统发生树, 基因型, 聚合酶链反应

Abstract:

Objective To determine the complete genomic sequences of 3 Dengue 2 virus (DEN-2) strains isolated in Guangdong province, China and to investigate their genotypes and sources. Methods The complete genomic sequences of 3 DEN-2 strains (GD09/93, GD05/98, and GD19/2001) from Guangdong province were established by RT-PCR amplification. The phylogenetic tree of DEN-2 was constructed by Kimura method. Results The complete genomic sequences of 3 DEN-2 strains were 10 723 nucleotides (nt) in length and contained a single long open reading frame (ORF) of 10 173 nucleotides (97-10 269 nt), encoding 3391 amino acids. The ORF encoded structural and non-structural proteins, flanked by 5' and 3' non-coding regions. Comparing GD05/98 with GD09/93, GD05/98 with GD19/2001, and GD09/93 with GD19/2001, the nucleotide sequence homologies were 93.3%, 92.4%, and 97.6%, respectively, and the deduced amino acid sequence homologies were 96.7%, 96.5%, and 98.5%, respectively. Conclusion The DEN-2 strains are pathogenic in suckling mice. Compared with DEN-2-04 strain (nonpathogenic in suckling mice), there are 18 amino acid substitutions that confer changes in charge or polarity. The charge changes at PrM-134, NS2A-153, and NS4B-102 have a relatively strong impact on the antigenicity. GD05/98 strain falls within the groupⅡ comprising Thailand strains, while GD09/93 and GD19/2001 strains fall within the group Ⅳ comprising strains from Indonesia, Australian and Taiwan. There are different DEN genotypes in China, and one genotype of DEN-2 may be transmitted in different periods.

Key words: Dengue virus, Complete genomic sequence, Phylogenetic tree, Genotype, Polymerase chain reaction

中图分类号:

R373.3⁺3

白志军, 张培, 洪文艳, 刘建伟, 狄飚, 杨智聪, 任瑞文, 方美玉, 林立辉. 广东省流行的3株登革热2型病毒基因组全序列测定及分析[J]. 中国媒介生物学及控制杂志, 2013, 24(5): 392-396.

BAI Zhi-jun, ZHANG Pei, HONG Wen-yan, LIU Jian-wei, DI Biao, YANG Zhi-cong, REN Rui-wen, FANG Mei-yu, LIN Li-hui. Determination and analysis of complete genomic sequences of three Dengue 2 virus strains isolated in Guangdong province, China[J]. Chines Journal of Vector Biology and Control, 2013, 24(5): 392-396.

参考文献

[1] Rico-Hesse R. Molecular evolution and distribution of Dengue viruses type 1 and 2 in nature
[J]. Virology,1990,174(2):479-493.

[2] Weaver SC,Vasilakis N. Molecular evolution of Dengue viruses: contributions of phylogenetics to understanding the history and epidemiology of the preeminent arboviral disease
[J]. Infect Genet Evo,2009,9(4):523-540.

[3] Vasilakis N,Weaver SC. The history and evolution of human dengue emergence
[J]. Adv Virus Res,2008,2:1-76.

[4] Bhaumik S. Study estimates 390 million dengue cases a year in world,with India having highest burden
[J]. BMJ,2013,346:f2339.

[5] 陈文新. 细菌系统发育
[J]. 微生物学报,1998,38(3):240.

[6] 胡志君,杨敬,赵卫,等. 我国两株登革热2型病毒基因组的全序列分析
[J]. 中国病毒学,2002,17(1):17-21.

[7] Thant KZ, Morita K, Igarashi A. Sequences of E/NSl gene junction from four Dengue-2 viruses of northeastern Thailand and their evolutionary relationships with other Dengue-2 viruses
[J]. Microbio Immunol,1995,39:581-590.

[8] Bray M, Men R, Tokimatsr I, et al. Genetic determinants responsible for acquisition of Dengue type 2 virus mouse neurovirulence
[J]. J Viml,1998,72:1647-1651.

[9] Lok SM, Ng ML, Aaskov J. Amino acid and phenotypic changes in Dengue 2 virus associated with escape from neutralization by IgM antibody
[J]. J Med Virol,2001,65(2):315-323.

[10] Despres P, Frenkiel MP, Deubel V. Differences between cell membrane fusion activities of two Dengue type-1 isolates reflect modifications of viral structure
[J]. Virology,1993,196(1):209-219.

[11] Holmes EC, Worobey M, Rambaut A. Phylogenetic evidence for recombination in Dengue virus
[J]. Mol Biol Evol,2000,16(3): 405-409.

[12] Rodriguez-Roche R, Sanchez L, Burgher Y, et al. Virus role during intraepidemic increase in dengue disease severity
[J]. Vector Borne Zoonotic Dis,2011,11(6):675-681.

[13] Wong SS, Abd-Jamil J, Abubakar S. Antibody neutralization and viral virulence in recurring Dengue virus type 2 outbreaks
[J]. Viral Immunol,2007,20(3):359-368.

[14] Grant D, Tan GK, Qing M, et al. A single amino acid in nonstructural protein NS4B confers virulence to Dengue virus in AG129 mice through enhancement of viral RNA synthesis
[J]. J Virol,2011,85(15):7775-7787.

[15] 曹丹. 登革病毒基因组碱基变异与毒力的关系
[J]. 热带医学杂志,2007,7(1):98-101.

[16] 赵卫,杨佩英. 登革病毒毒力研究进展
[J]. 中华实验和临床病毒学杂志,2001,15(2):197-199.

[17] 赵卫,张文炳. 登革病毒进化遗传学与毒力关系研究进展
[J]. 微生物学免疫学进展,2003,31(2):54-56.

[18] 张拥军,周朝晖,黄萌,等. 登革病毒的基因组分析及中国登革毒株的来源
[J]. 中国人兽共患病学报,2012,28(6):515-520.

[19] 白志军,和鹏,狄飚,等. 广州市2010年1～3型登革病毒E基因进化分析
[J]. 中华传染病杂志,2012,30(3):152-156.

[20] 秦鄂德,秦成峰,姜涛. 登革病毒和登革病毒病
[M]. 北京:科学出版社,2008:143-164.

广东省流行的3株登革热2型病毒基因组全序列测定及分析

Determination and analysis of complete genomic sequences of three Dengue 2 virus strains isolated in Guangdong province, China

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	南晓伟, 解新霞, 于红敏, 司晓艳, 陈继来, 张晨光, 张秀红. 内蒙古包头市2018年一起流行性乙型脑炎疫情的媒介蚊虫及感染虫媒病毒调查[J]. 中国媒介生物学及控制杂志, 2020, 31(6): 652-656.
[2]	胡挺松, 李应, 胡秋凌, 吴学林, 高丽芬, 李国伟, 王志勇, 胡海梅, 王意银, 张海林, 张富强. 云南省楚雄州恙虫病流行特征及其病原体东方体基因分型研究[J]. 中国媒介生物学及控制杂志, 2020, 31(3): 282-288.
[3]	段兴德, 何志海, 高子厚, 蒋宝贵, 龚正达, 张云, 邵宗体, 江佳富, 孙毅, 刘洪波, 姚明国, 王帆, 杜春红. 云南省边境地区卵形硬蜱中中华基因型伯氏疏螺旋体的检出和鉴定[J]. 中国媒介生物学及控制杂志, 2019, 30(5): 519-523.
[4]	刘维俊, 肖方震, 林思娴, 韩腾伟, 周淑姮, 邓艳琴. 福建省5县（市）鼠类感染伯氏疏螺旋体的调查研究[J]. 中国媒介生物学及控制杂志, 2019, 30(3): 320-323.
[5]	薛志静, 王君, 宋秀平, 刘小波, 郭玉红, 栗冬梅, 王雪霜, 刘起勇. 登革热病毒分子生物学特性及检测方法研究进展[J]. 中国媒介生物学及控制杂志, 2019, 30(2): 224-227.
[6]	杨瑞军, 黄世腾, 王晓光, 吕磊, 曹国平, 万圣, 叶承华, 陈旭富. 浙江省衢州市1例输入性登革热病例分子流行病学研究[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 445-447.
[7]	马敏, 马晓, 杨思嘉, 王桂安, 孙斌. 登革热病毒的垂直传递和伊蚊滞育研究进展[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 539-542.
[8]	刘晓娜, 吴能简, 吴崧霖, 陈景阳, 谢美莲, 王德全. 深圳市坪山新区2016年登革热媒介监测结果分析[J]. 中国媒介生物学及控制杂志, 2017, 28(3): 283-285.
[9]	王艳华, 毛玲玲, 彭遥, 孙英伟, 王子江, 张家勇, 张稷博, 田疆. 在辽宁省长角血蜱中检测出土拉弗朗西斯菌[J]. 中国媒介生物学及控制杂志, 2016, 27(6): 529-532.
[10]	刘红, 梁国栋. 新发虫媒病毒:基因A型版纳病毒全基因组序列扩增引物[J]. 中国媒介生物学及控制杂志, 2016, 27(6): 533-538.
[11]	吕燕宁, 李良辰, 窦相峰, 陈丽娟, 张秀春, 关增智, 孙玉兰, 黎新宇, 王全意. 2013年北京地区小兽中3种病原携带状况调查[J]. 中国媒介生物学及控制杂志, 2016, 27(4): 336-340.
[12]	姜进勇, 郭晓芳, 唐烨榕, 杨明东, 魏春, 周红宁. 云南省2004—2014年输入性登革热病例监测与防控对策分析[J]. 中国媒介生物学及控制杂志, 2016, 27(1): 5-8.
[13]	廖虹瑜, 蒋德勇, 母永贵, 杨小蓉. 巢式PCR及序列分析用于攀枝花市恙虫病东方体检测[J]. 中国媒介生物学及控制杂志, 2016, 27(1): 38-41.
[14]	段金花, 蔡松武, 吴军, 邹钦, 阴伟雄. 广东省蚊媒西尼罗病毒携带情况调查研究[J]. 中国媒介生物学及控制杂志, 2015, 26(6): 558-560.
[15]	孙炳欣, 孙宇, 沈博, 吴静. 长春市鼠类携带汉坦病毒的基因型分析[J]. 中国媒介生物学及控制杂志, 2015, 26(6): 600-604.