基于rDNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究(双翅目：蚊科)

doi:10.11853/j.issn.1003.4692.2013.05.002

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (5): 382-388.DOI: 10.11853/j.issn.1003.4692.2013.05.002

基于rDNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究(双翅目：蚊科)

马颖, 马雅军, 林琳, 王琰

第二军医大学热带传染病学教研室, 上海 200433

收稿日期:2013-05-20 出版日期:2013-10-20 发布日期:2013-10-20
通讯作者: 马雅军, Email: yajun_ma@163.com
作者简介:马颖(1983- ),女,动物学硕士,主要从事媒介生物学研究工作。Email: m_y_1001@yahoo.cn
基金资助:
国家自然科学基金委员会-云南省人民政府联合基金(U0932604)

Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles (Diptera：Culicidae) in China：based on rDNA-ITS2 sequences

MA Ying, MA Ya-jun, LIN Lin, WANG Yan

Department of Tropical Infectious Diseases, Second Military Medical University, Shanghai 200433, China

Received:2013-05-20 Online:2013-10-20 Published:2013-10-20
Supported by:
Supported by the National Natural Science Foundation of China-Yunnan Joint Fund (No. U0932604)

摘要/Abstract

摘要：

目的阐明中国按蚊属按蚊亚属蚊种的亲缘关系。方法根据核糖体DNA第2内转录间隔区(rDNA-ITS2)序列,采用邻接法(NJ)、最大简约法(MP)、最小进化法(ME)和最大似然法(ML),对采自中国的按蚊亚属22种(分子型)蚊虫进行系统发育关系重建。结果 rDNA-ITS2序列的长度范围为340～1754 bp,种内的p遗传距离均小于0.004,种间为0.009～0.420,平均值为0.224。各种方法重建的系统发育树均显示分为3大支,分别对应赫坎按蚊种团和须喙按蚊种团各一支,以及林氏按蚊与五斑按蚊种团聚为的一支,三者为并系关系;赫坎按蚊种团成员种分为主要的4支,其种间关系与形态分类结果一致,但分子型spLL1和spLL2的聚类位置不稳定。结论重建的分子系统发育树客观地阐明了中国按蚊属按蚊亚属各种团及蚊种之间的亲缘关系,ITS2序列具有足够的信息贡献量。

关键词: 按蚊属, 按蚊亚属, 核糖体DNA第2内转录间隔区, 系统发育

Abstract:

Objective To clarify the interspecific phylogenetic relationship among the species of genus Anopheles subgenus Anopheles in China. Methods The phylogenetic relationship among 22 species (molecular forms) of subgenus Anopheles collected from China was reconstructed based on ribosomal DNA second internal transcribed spacer (rDNA-ITS2) sequences using neighbour joining, maximum parsimony, minimum evolution and maximum likelihood methods. Results The length range of rDNA-ITS2 was 340-1754 bp; the intraspecific genetic distances (p distance) were below 0.004, and the range of pairwise genetic distances among Anopheles mosquitoes was 0.009-0.420 (mean 0.224). All the reconstructed phylogeny trees showed that there were three clades, i.e., An. hyrcanus group, An. barbirostris group and An. lindesayi with An. maculipennis group and that the group clades were paraphyletic. There were four major clusters in An. hyrcanus group, and the interspecific relationship in each cluster was the same as the morphological taxonomy. However, the cluster positions of spLL1 and spLL2 were unstable. Conclusion The molecular phylogenetic tree reconstructed in this study objectively shows the interspecific phylogenetic relationship among the groups and species of genus Anopheles subgenus Anopheles in China. The rDNA-ITS2 sequences have sufficient contribution to the reconstruction of phylogeny of Anopheles species.

Key words: Genus Anopheles, Subgenus Anopheles, Ribosomal DNA second internal transcribed spacer, Phylogeny

中图分类号:

R384.1

马颖, 马雅军, 林琳, 王琰. 基于rDNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究(双翅目：蚊科)[J]. 中国媒介生物学及控制杂志, 2013, 24(5): 382-388.

MA Ying, MA Ya-jun, LIN Lin, WANG Yan. Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles (Diptera：Culicidae) in China：based on rDNA-ITS2 sequences[J]. Chines Journal of Vector Biology and Control, 2013, 24(5): 382-388.

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基于rDNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究(双翅目：蚊科)

Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles (Diptera：Culicidae) in China：based on rDNA-ITS2 sequences

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