中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (3): 262-264.

• 调查研究 • 上一篇    下一篇

深圳市家蝇和大头金蝇抗药性监测研究

刘阳1, 梁焯南1, 贾凤龙2, 张韶华1   

  1. 1. 深圳市疾病预防控制中心, 广东 深圳 518055;
    2. 中山大学生命科学学院昆虫学研究所
  • 收稿日期:2012-11-29 出版日期:2013-06-20 发布日期:2013-06-20
  • 通讯作者: 张韶华,Email: zhshh999@tom.com
  • 作者简介:刘阳(1986- ),男,硕士,主要从事媒介生物控制工作。Email: zsuinsect@163.com

Study on insecticide resistance of Musca domestica and Chrysomya megacephala in Shenzhen, China

LIU Yang1, LIANG Zhuo-nan1, JIA Feng-long2, ZHANG Shao-hua1   

  1. 1. Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, Guangdong Province, China;
    2. Institute of Entomology, the School of Life Science of Sun Yat-Sen University
  • Received:2012-11-29 Online:2013-06-20 Published:2013-06-20

摘要:

目的 了解近年来深圳市家蝇的抗药性发展状况,探讨大头金蝇对几种常用杀虫剂的抗性,为防制工作提供科学依据。方法 采集深圳市区家蝇及大头金蝇,挑选实验室饲养1~2代后的雌性成虫,采用微量点滴法测定其对溴氰菊酯、高效氯氰菊酯、氯菊酯、胺菊酯、DDVP和仲丁威6种杀虫剂的LD5(0 μl/虫)。结果 家蝇对溴氰菊酯、氯菊酯、胺菊酯、DDVP、仲丁威的LD50分别为0.024 819、0.092 076、0.649 075、0.065 610和5.008 337 μg/虫,与1998年的抗性监测结果相比,分别增长8.27、7.25、3.45、0.11和0.54倍;大头金蝇对溴氰菊酯、高效氯氰菊酯、氯菊酯、胺菊酯、DDVP和仲丁威的LD50分别为0.271 852、0.318 642、0.174 697、0.657 885、0.086 490和1.743 459 μg/虫。结论 家蝇对溴氰菊酯、氯菊酯、胺菊酯的抗性水平均有不同程度升高,但抗性增长速度较为缓慢,对DDVP与仲丁威的抗性呈现下降趋势;大头金蝇的抗性测定结果与家蝇有差异,需改进实验方法并制定相应的抗性级别标准。

关键词: 家蝇, 大头金蝇, 抗药性

Abstract:

Objective To investigate the insecticide resistance of Musca domestica in recent years and the resistance of Chrysomya megacephala to several common insecticides in Shenzhen, China, and to provide a scientific basis for the control of flies. Methods M. domestica and C. megacephala were collected in the urban area of Shenzhen and raised in the laboratory for 1-2 generations. Topical application was used to determine the median lethal doses (LD50) (μg/insect) of deltamethrin, beta-cypermethrin, permethrin, tetramethrin, DDVP, and fenobucarb for M. domestica and C. megacephala. Results For M. domestica, the LD50 of deltamethrin, permethrin, tetramethrin, DDVP, and fenobucarb were 0.024 819, 0.092 076, 0.649 075, 0.065 610, and 5.008 337 μg/insect, respectively, increased by 8.27, 7.25, 3.45, 0.11, and 0.54 times compared with the resistance monitoring data in 1998. For C. megacephala, the LD50 of deltamethrin, beta-cypermethrin, permethrin, tetramethrin, DDVP, and fenobucarb were 0.271 852, 0.318 642, 0.174 697, 0.657 885, 0.086 490, and 1.743 459 μg/insect, respectively. Conclusion The resistance of M. domestica to deltamethrin, permethrin, and tetramethrin increases slowly, while its resistance to DDVP and fenobucarb exhibits a declining trend. The results of resistance test for C. megacephala are different from those for M. domestica, and it is necessary to improve experimental methods and establish the standard for resistance levels.

Key words: Musca domestica, Chrysomya megacephala, Insecticide resistance

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