脉冲场凝胶电泳技术简介及其在细菌分子分型中的应用

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (2): 182-185.

脉冲场凝胶电泳技术简介及其在细菌分子分型中的应用

叶蕊¹, 石丽媛², 王鹏², 宋志忠^1,2

1 大理学院公共卫生学院,云南大理 671000;
2 云南省地方病防治所,云南省鼠疫防控技术重点实验室,云南大理 671000

收稿日期:2012-11-16 出版日期:2013-04-20 发布日期:2013-04-20
通讯作者: 宋志忠
基金资助:
国家自然科学基金(81160354);卫生部行业科研专项(201202021);云南省应用基础研究(2011FB134)

Brief introduction of pulsed-field gel electrophoresis and its application in bacterial molecular typing

YE Rui¹, SHI Li-yuan², WANG Peng², SONG Zhi-zhong^1,2

1 Dali University, School of Public Health, Dali 671000, Yunnan Province, China;
2 Provincial Key Laboratory for Plague Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, Yunnan Province, China

Received:2012-11-16 Online:2013-04-20 Published:2013-04-20
Supported by:
Supported by the National Natural Science Foundation of China(No. 81160354), Ministry of Industry Fund(No. 201202021) and Yunnan Applied Basic Research(No. 2011FB134)

摘要/Abstract

摘要： 目前可用于细菌分子分型的方法较多,脉冲场凝胶电泳(PFGE)技术以其重复性好、分辨力强的优势被作为细菌分子分型的“金标准”广泛应用;利用PFGE技术对细菌进行分型并分析细菌间分型结果的差异,可以有效地对疾病进行监测,追溯疾病的感染来源,明确感染途径,追溯其遗传特征,为细菌性疾病的预防与控制提供分子生物学证据.

关键词: 脉冲场凝胶电泳, 细菌, 分子分型, 应用

Abstract: At present, there are many methods for bacterial molecular typing. Pulsed-field gel electrophoresis (PFGE) is widely used as the“gold standard”for bacterial molecular typing due to its good repeatability and high resolution. Using PFGE for bacterial molecular typing and bacterial type differentiation, we can effectively monitor diseases, trace the source of infection, specify the route of infection, and determine the genetic characteristics of bacteria, thus providing a molecular biological basis for the prevention and control of bacterial diseases.

Key words: Pulsed-field gel electrophoresis, Bacteria, Molecular typing, Application

中图分类号:

R331

叶蕊, 石丽媛, 王鹏, 宋志忠. 脉冲场凝胶电泳技术简介及其在细菌分子分型中的应用[J]. 中国媒介生物学及控制杂志, 2013, 24(2): 182-185.

YE Rui, SHI Li-yuan, WANG Peng, SONG Zhi-zhong. Brief introduction of pulsed-field gel electrophoresis and its application in bacterial molecular typing[J]. Chines Journal of Vector Biology and Control, 2013, 24(2): 182-185.

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