德国小蠊抗药性与乙酰胆碱酯酶、谷胱甘肽S-转移酶和超氧化物歧化酶活性关系的研究

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (2): 135-137.

德国小蠊抗药性与乙酰胆碱酯酶、谷胱甘肽S-转移酶和超氧化物歧化酶活性关系的研究

王学军^1,2, 康殿民^1,2, 张凡³, 赵志刚^1,2

1 山东省疾病预防控制中心病媒生物防制所,山东济南 250014;
2 山东省传染病预防控制重点实验室,山东济南 250014;
3 山东师范大学生命科学院

收稿日期:2012-12-14 出版日期:2013-04-20 发布日期:2013-04-20
基金资助:
山东省医药卫生科技发展计划项目(2009HW055)

Study on relationship between insecticide resistance and activities of acetylcholinesterase, glutathione S-transferase, and superoxide dismutase in Blattella germanica

WANG Xue-jun^1,2, KANG Dian-min^1,2, ZHANG Fan³, ZHAO Zhi-gang^1,2

1 Shandong Center for Disease Control and Prevention, Jinan 250014, Shandong Province, China;
2 Shandong Key Laboratory for Infectious Diseases Prevention and Control, Jinan 250014, Shandong Province, China;
3 Shandong Normal University, College of Life Sciences

Received:2012-12-14 Online:2013-04-20 Published:2013-04-20
Supported by:
Supported by the Development Plan Project of Shandong Province Science and Technology of Medical and Health(No. 2009HW055)

摘要/Abstract

摘要： 目的了解山东省不同地理种群德国小蠊乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GSTs)和超氧化物歧化酶(SOD)的活性,分析它们与抗药性的关系,探讨德国小蠊抗药性发生的生化机制及相关酶活性作为抗药性监测指标的可行性.方法采用分光光度计法,测定不同地理种群德国小蠊AChE、GSTs和SOD的活性.结果济南、淄博、东营、德州市4个不同地理种群德国小蠊的GSTs、AChE活性较敏感品系均有不同程度升高,GSTs、AChE活性相对比值分别为1.11～1.53、2.44～3.46,且差异有统计学意义(P ＜ 0.05),SOD活性相对比值为1.00～1.12,与敏感品系比较差异无统计学意义.结论德国小蠊野外种群GSTs、AChE活性上的差异表明GSTs、AChE与德国小蠊抗药性的产生和发展密切相关,其活性相对比值可作为德国小蠊抗药性的监测指标,而SOD与抗药性的关系不显著,其活性相对比值仅能作为德国小蠊抗药性的参考指标.

关键词: 德国小蠊, 抗药性, 酶活性

Abstract: Objective To investigate the activities of acetylcholinesterase (AChE), glutathione S-transferase (GSTs), and superoxide dismutase (SOD) in different field populations of Blattella germanica in Shandong province, and analyze their relationship with insecticide resistance, and to study the biochemical mechanism by which B. germanica develops insecticide resistance and explore the feasibility of using the enzymatic activities as the monitoring indices for insecticide resistance. Methods The activities of AChE, GSTs, and SOD in different field populations of B. germanica were measured by spectrophotometry. Results The activities of GSTs and AChE in the B. germanica from Jinan, Zibo, Dongying, and Dezhou were significantly higher than those in the sensitive strain, with relative ratios of 1.11-1.53 and 2.44-3.46, respectively (P ＜ 0.05). There was a positive correlation between GSTs and AChE activities and the insecticide resistance of B. germanica. On the other hand, there were no significant differences in SOD activity between these field populations and the sensitive strain, with a relative ratio of 1.00-1.12. Conclusion The field populations of B. germanica have higher activities of GSTs and AChE than the sensitive strain, which suggests that GSTs and AChE are involved in the development of insecticide resistance in B. germanica and closely related to this process. Therefore, the relative activities of GSTs and AChE may be used as the monitoring indices for the insecticide resistance of B. germanica. There is no significant correlation between SOD activity and insecticide resistance in B. germanica, so the relative activity of SOD can only be used as a reference index for the insecticide resistance of B. germanica.

Key words: Blattella germanica, Insecticide resistance, Enzymatic activities

中图分类号:

王学军, 康殿民, 张凡, 赵志刚. 德国小蠊抗药性与乙酰胆碱酯酶、谷胱甘肽S-转移酶和超氧化物歧化酶活性关系的研究[J]. 中国媒介生物学及控制杂志, 2013, 24(2): 135-137.

WANG Xue-jun, KANG Dian-min, ZHANG Fan, ZHAO Zhi-gang. Study on relationship between insecticide resistance and activities of acetylcholinesterase, glutathione S-transferase, and superoxide dismutase in Blattella germanica[J]. Chines Journal of Vector Biology and Control, 2013, 24(2): 135-137.

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