巢式PCR用于疑似莱姆病患者血清标本检测的研究

中国媒介生物学及控制杂志 ›› 2013, Vol. 24 ›› Issue (1): 8-10.

巢式PCR用于疑似莱姆病患者血清标本检测的研究

张刘丽^1,2, 侯学霞², 耿震², 霍秋波³, 郝琴², 万康林², 楼永良¹

1. 温州医学院检验医学院,浙江温州325035;
2. 中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室,北京102206;
3. 黑龙江省牡丹江林业中心医院

收稿日期:2012-08-24 出版日期:2013-02-20 发布日期:2013-02-20
通讯作者: 楼永良,Email:lyl10282004@yahoo.com.cn;郝琴,Email:haoqin@icdc.cn
基金资助:
国家“十二五”传染病重大专项(2011ZX10004-001);国家自然科学基金(81171611)

Nested PCR for testing serum samples of suspected Lyme disease patients

ZHANG Liu-li^1,2, HOU Xue-xia², GENG Zhen², HUO Qiu-bo³, HAO Qin², WAN Kang-lin², LOU Yong-liang¹

1. School of Laboratory Medicine Wenzhou Medical College, Wenzhou 325035, Zhejiang Province, China;
2. State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
3. Mudanjing Forestry Center Hospital

Received:2012-08-24 Online:2013-02-20 Published:2013-02-20
Supported by:
Supported by the National Science and Technology Major Project for Infectious Diseases in China(No. 2011ZX10004-001) and National Natural Science Foundation of China(No. 81171611)

摘要/Abstract

摘要： 目的研究rrf(5S)～rrl(23S) rRNA基因间隔区巢式PCR用于疑似莱姆病患者血清标本的检测效果。方法收集疑似莱姆病患者血清标本及其流行病学和临床资料,提取DNA进行巢式PCR、普通PCR检测。结果共收集102份疑似莱姆病患者血清,巢式PCR检测阳性39例,阳性率为38.23%,其中蜱叮咬时间在2个月内的为33例,占84.62%;普通PCR只有1份阳性,阳性率为0.98%,远低于巢式PCR(38.23%)。结论 rrf(5S)～rrl(23S) rRNA基因间隔区巢式PCR可用于疑似莱姆病患者血清标本检测,从病原学角度支持莱姆病的诊断。

关键词: 莱姆病, 巢式PCR, rrf(5S)～rrl(23S)rRNA基因间隔区

Abstract: Objective To study the effect of nested polymerase chain reaction (PCR) of rrf(5S)-rrl(23S) intergenic spacer rRNA in serum for diagnosing suspected Lyme disease patients. Methods Serum samples, epidemiological data, and clinical information of suspected Lyme disease patients were collected. Then, DNA were extracted from the serums and detected by nested PCR and conventional PCR. Results Out of 102 serum samples collected from suspected Lyme disease patients, 39 positive ones were detected by nested PCR, with a positive rate of 38.23%; the bite time of 33(84.62%) was within two months. Only one was positive by conventional PCR, with a positive rate of 0.98%, far less than nested PCR(38.23%). Conclusion For suspected Lyme disease patients' serum samples, nested PCR of rrf(5S)-rrl(23S) intergenic spacer rRNA could be a method to diagnose Lyme disease etiologically.

Key words: Lyme disease, Nested polymerase chain reaction, rrf(5S)-rrl(23S) intergenic spacer rRNA

中图分类号:

R377

张刘丽, 侯学霞, 耿震, 霍秋波, 郝琴, 万康林, 楼永良. 巢式PCR用于疑似莱姆病患者血清标本检测的研究[J]. 中国媒介生物学及控制杂志, 2013, 24(1): 8-10.

ZHANG Liu-li, HOU Xue-xia, GENG Zhen, HUO Qiu-bo, HAO Qin, WAN Kang-lin, LOU Yong-liang. Nested PCR for testing serum samples of suspected Lyme disease patients[J]. Chines Journal of Vector Biology and Control, 2013, 24(1): 8-10.

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