kdr突变与淡色库蚊对溴氰菊酯抗性关系研究

中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (6): 516-518,521.

kdr突变与淡色库蚊对溴氰菊酯抗性关系研究

刘宏美, 刘丽娟, 黄晓丹, 代玉华, 程鹏, 王海防, 赵玉强, 王怀位, 公茂庆

山东省医学科学院, 山东省寄生虫病防治研究所, 山东济宁 272033

收稿日期:2012-06-27 出版日期:2012-12-20 发布日期:2012-12-20
通讯作者: 公茂庆,Email:maoqingg@yahoo.com
基金资助:
国家自然科学基金(81271877); 山东省国际科技合作项目(LKH09-187-06); 山东省自然科学基金(ZR2009CQ029, ZR2010LH020); 山东省优秀中青年科学家科研奖励基金(bs2011SW032)

Association of kdr gene mutation with deltamethrin resistance in Culex pipiens pallens

LIU Hong-mei, LIU Li-juan, HUANG Xiao-dan, DAI Yu-hua, CHENG Peng, WANG Hai-fang, ZHAO Yu-qiang, WANG Huai-wei, GONG Mao-qing

Shandong Academy of Medical Sciences, Shandong Institute of Parasitic Diseases, Jining 272033, Shandong Province, China

Received:2012-06-27 Online:2012-12-20 Published:2012-12-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 81271877), the International Science and Technological Cooperation Project of Shandong Province (No. LKH09-187-06), the Natural Science Foundation of Shandong Province (No. ZR2009CQ029, ZR2010LH020) and Excellent Young Scientist Research Award Projects of Shandong (No. bs2011SW032)

摘要/Abstract

摘要： 目的了解山东省不同地区淡色库蚊成蚊对溴氰菊酯抗性与kdr等位基因的突变关联情况。方法采用AS-PCR(allele-specific PCR)技术, 检测不同种群淡色库蚊成蚊的kdr等位基因频率。结果成蚊接触筒检测济南、济宁和青岛地区的淡色库蚊雌蚊, 其死亡率分别为39.62%、37.25%和83.81%;通过回归分析AS-PCR检测的kdr等位基因频率结果, 显示不同地区淡色库蚊对溴氰菊酯抗性L1014F型和L1014S型突变表型与kdr等位基因频率有明显的相关性。结论淡色库蚊kdr基因突变与溴氰菊酯抗药性表型呈正相关, AS-PCR方法对于检测kdr等位基因的可行性亟待验证性研究。

关键词: 淡色库蚊, 抗药性, 溴氰菊酯

Abstract: Objective To investigate the association of kdr gene mutation with deltamethrin resistance in adult Culex pipiens pallens from different regions in Shandong province, China. Methods The frequency of each kdr allele in different populations of adult Cx. pipiens pallens was determined by allele-specific PCR (AS-PCR). Results The mortality rates of female Cx. pipiens pallens from Jinan, Jining, and Qingdao in Shandong were 39.62%, 37.25%, and 83.81%, respectively, as measured by adult mosquito contact tube. There was significant correlation between deltamethrin resistance mutation phenotypes of L1014F and L1014S and frequencies of kdr alleles in Cx. pipiens pallens from different regions, according to the regression analysis of AS-PCR results. Conclusion Mutation of kdr gene is positively correlated with deltamethrin resistance phenotypes in Cx. pipiens pallens. The feasibility of the AS-PCR method for detection of kdr allele needs further validation.

Key words: Culex pipiens pallens, Insecticide resistance, Deltamethrin

中图分类号:

刘宏美, 刘丽娟, 黄晓丹, 代玉华, 程鹏, 王海防, 赵玉强, 王怀位, 公茂庆. kdr突变与淡色库蚊对溴氰菊酯抗性关系研究[J]. 中国媒介生物学及控制杂志, 2012, 23(6): 516-518,521.

LIU Hong-mei, LIU Li-juan, HUANG Xiao-dan, DAI Yu-hua, CHENG Peng, WANG Hai-fang, ZHAO Yu-qiang, WANG Huai-wei, GONG Mao-qing. Association of kdr gene mutation with deltamethrin resistance in Culex pipiens pallens[J]. Chines Journal of Vector Biology and Control, 2012, 23(6): 516-518,521.

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