浦东机场口岸蝇类携带病原菌种类及16S rRNA鉴定

中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (6): 506-511.

浦东机场口岸蝇类携带病原菌种类及16S rRNA鉴定

陈拓¹, 曹晓梅², 邓耀华³, 房健慧², 李飞¹

1. 南京农业大学, 江苏南京 210095;
2. 中国检验检疫科学研究院, 北京 100123;
3. 上海出入境检验检疫局

收稿日期:2012-07-16 出版日期:2012-12-20 发布日期:2012-12-20
通讯作者: 李飞,Email:lifei@njau.edu.cn;曹晓梅,Email:cxm910@126.com
基金资助:
国家质检总局科技计划项目(2011IK153); 中国检验检疫科学研究院所基金(2011JK007)

Species and 16S rRNA identification of fly-borne pathogens in Shanghai Pudong International Airport

CHEN Tuo¹, CAO Xiao-mei², DENG Yao-hua³, FANG Jian-hui², LI Fei¹

1. Nanjing Agricultural College, Nanjing 210095, Jiangsu Province, China;
2. China Inspection and Quarantine Science Research Institute, Beijing 100123, China;
3. Shanghai Entry-Exit Inspection and Quarantine Bureau

Received:2012-07-16 Online:2012-12-20 Published:2012-12-20
Supported by:
Supported by the Science and Technology Projects of the State General Administration of Quality Supervision, Inspection and Quarantine of China (No. 2011IK153)

摘要/Abstract

摘要： 目的确定口岸蝇类携带病原菌种类及分布。方法将蝇类体表、体内细菌分离培养, 单克隆菌株经Vitek 2 Compact微生物自动分析及16S rRNA基因序列比对。结果在7种蝇体表检出18种菌, 其中致病菌2种, 条件致病菌14种, 包括1株国内未报道的条件致病菌株Wohlfahrtiimonas chitiniclastica;蝇体内检出14种菌, 其中致病菌1种, 条件致病菌12种;携带菌种类最多的是酱麻蝇, 其次为亮绿蝇。结论口岸蝇体携带病原菌的调查, 有利于对新型病原微生物入侵的鉴别及应对突发公共卫生事件。

关键词: 浦东机场, 口岸, 蝇类, 病原菌, 16S rRNA, 鉴定

Abstract: Objective To investigate the species and distributions of fly-borne pathogens in Shanghai Pudong International Airport. Methods The bacteria on the body surfaces and inside the bodies of flies were isolated and cultured. The monoclonal strains were subject to automatic analysis using Vitek 2 Compact and 16S rRNA sequence alignment. Results Eighteen species of bacteria (2 pathogens and 14 opportunistic pathogens including Wohlfahrtiimonas chitiniclastica that had not been reported in China) were detected on the body surfaces of 7 species of flies, Fourteen species of bacteria (1 pathogens and 12 opportunistic pathogens) were detected inside the bodies of the flies. The fly that carried the most species of bacteria was Sarcophaga misera, followed by Lucilia illustris. Conclusion The investigation of fly-borne pathogens in port will be in favor of the identification of new pathogen species and response to public health emergencies.

Key words: Pudong International Airport, Port, Fly, Pathogen, 16S rRNA, Identification

中图分类号:

R384.2
R372

陈拓, 曹晓梅, 邓耀华, 房健慧, 李飞. 浦东机场口岸蝇类携带病原菌种类及16S rRNA鉴定[J]. 中国媒介生物学及控制杂志, 2012, 23(6): 506-511.

CHEN Tuo, CAO Xiao-mei, DENG Yao-hua, FANG Jian-hui, LI Fei. Species and 16S rRNA identification of fly-borne pathogens in Shanghai Pudong International Airport[J]. Chines Journal of Vector Biology and Control, 2012, 23(6): 506-511.

参考文献

[1] Becker K, Harmsen D, Mellmann A, et al. Development and evaluation of quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species[J]. J Clin Microbiol, 2004, 42(11):4988-4995.
[2] Bosshard PP, Zbinden R, Abels S, et al. 16S rRNA gene sequencing versus the API 20 NE System and the Vitek 2 ID-GNB Card for identification of nonfermenting gram-negative bacteria in the clinical laboratory[J]. J Clin Microbiol, 2006, 44(4):1359-1366.
[3] von Eiff C, Peters G, Becker K. The small colony variant (SCV) concept-the role of staphylococcal SCVs in persistent infections[J]. Injury, 2006, 37 Suppl 2:S26-33.
[4] von Eiff C, Vaudaux P, Kahl BC, et al. Bloodstream infections caused by small-colony variants of coagulase-negative staphylococci following pacemaker implantation[J]. Clin Infect Dis, 1999, 29(4):932-934.
[5] Heikens E, Fleer A, Paauw A, et al. Comparison of genotypic and phenotypic methods for species-level identification of clinical isolates of coagulase-negative staphylococci[J]. J Clin Microbiol, 2005, 43(5):2286-2290.
[6] Clarridge JE 3rd. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious disease[J]. Clin Microbiol Rev, 2004, 17(4):840-862.
[7] Woo PC, Ng KH, Lau SK, et al. Usefulness of MicroSeq 500 16S ribosomal DNA-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles[J]. J Clin Microbiol, 2003, 41(5):1996-2001.
[8] Fontana C, Favaro M, Pelliccioni M, et al. Use of the MicroSeq 500 16S rRNA gene-based sequencing for identification of bacterial isolates that commercial automated systems failed to identify correctly[J]. J Clin Microbiol, 2005, 43(2):615-619.
[9] Carretto E, Barbarini D, Couto I, et al. Identification of coagulase-negative staphylococci other than Staphylococcus epidermidis by automated ribotyping[J]. Clin Microbiol Infect, 2005, 11(3):177-184.
[10] Layer F, Ghebremedhin B, Moder KA, et al. Comparative study using various methods for identification of Staphylococcus species in clinical specimens[J]. Clin Microbiol, 2006, 44(8):2824-2830.
[11] Jones SW, Franceseoni SC. DNA assays for detection, idcntification, and individualization of select agent microorganisms[J]. Croat Mcd J, 2005, 46(4):522-529.
[12] Noller HF, Hoang L. The 30S ribosomal P site: a function of 16S rRNA[J]. FEBS Lett, 2005, 579(4):855-858.
[13] Kibe R, Sakamoto M, Hayashi H, et al. Maturation of the murine cecal microbiota as revealed by terminal restriction fragment length polymorphism and 16S rRNA gene clone libraries[J]. FEMS Microbiol Lett, 2004, 235(1):139-146.
[14] Petrosino JF, Highlander S, Luna RA, et al. Metagenomic pyrosequencing and microbial identification[J]. Clin Chem, 2009, 55(5):856-866.
[15] Peterson DA, Frank DN, Pace NR, et al. Metagenomic approaches for defining the pathogenesis of inflammatory bowel diseases[J]. Cell Host Microbe, 2008, 3(6):417-427.
[16] Sönksen UW, Christensen JJ, Nielsen L, et al. Fastidious Gram-Negatives: identification by the Vitek 2 Neisseria-Haemophilus card and by partial 16S rRNA gene sequencing analysis[J]. Open Microbiol J, 2010, 4:123-131.
[17] Zbinden A, Böttger EC, Bosshard PP, et al. Evaluation of the colorimetric Vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16S rRNA gene sequencing[J]. J Clin Microbiol, 2007, 45(7):2270-2273.
[18] 陈佳木, 李亚伦, 高思维, 等. 马尾口岸蝇类生物多样性和携带病原微生物情况的研究[J]. 检验检疫学刊, 2009, 19(6):21-27.
[19] Tóth EM, Schumann P, Borsodi AK, et al. Wohlfahrtiimonas chitiniclastica gen. nov., sp. nov., a new gammaproteobacterium isolated from Wohlfahrtia magnifica (Diptera: Sarcophagidae)[J]. Int J Syst Evol Microbiol, 2008, 58(4):976-981.
[20] Rebaudet S, Genot S, Renvoise A, et al. Wohlfahrtiimonas chitiniclastica bacteremia in homeless woman[J]. Emerg Infect Dis, 2009, 15(6):985-986.
[21] Almuzara MN, Palombarani S, Tuduri A, et al. First case of fulminant sepsis due to Wohlfahrtiimonas chitiniclastica[J]. J Clin Microbiol, 2011, 49(6):2333-2335.