中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (4): 306-309.

• 论著 • 上一篇    下一篇

陕西省鼠疫自然疫源地新纪录鼠种的鉴定

安翠红1, 陈宝宝1, 范锁平1, 孙养信1, 李伟华2, 鲁亮3, 李贵昌3   

  1. 1 陕西省疾病预防控制中心鼠布生防科, 陕西西安 710054;
    2 定边县地方病防治研究所;
    3 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室
  • 收稿日期:2012-02-29 出版日期:2012-08-20 发布日期:2012-08-20
  • 通讯作者: 孙养信,Email: sxpco@126.com
  • 基金资助:
    陕西省科学技术研究发展计划项目(2012K16-12-03)

Identification of newly-recorded Microtus fortis species in the plaque foci of Shaanxi province

AN Cui-hong1, CHEN Bao-bao1, FAN Suo-ping1, SUN Yang-xin1, LI Wei-hua2, LU Liang3, LI Gui-chang3   

  1. 1 Shaanxi Center for Disease Control and Prevention, Xi’an 710054, Shaanxi Province, China;
    2 Dingbian Center for Disease Control and Prevention;
    3 State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
  • Received:2012-02-29 Online:2012-08-20 Published:2012-08-20
  • Supported by:
    Supported by the Science and Technology Researoh and Development Projects of Shaanxi Province(No. 2012K16-12-03)

摘要: 目的 对陕西省鼠疫疫源地定边县鼠疫监测中新纪录鼠种进行分类鉴定。方法 运用形态学方法及DNA条形码技术进行鉴定分析。结果 体长120~140 mm,体重41.75~71.58 g,颅全长<32 mm,尾长超过体长1/3,全身灰褐色,腹面沙黄色;线粒体细胞色素C氧化酶Ⅰ亚基基因序列与其他地区东方田鼠标本种内遗传距离最大为1%。结论 定边县鼠疫疫区鄂尔多斯高原荒漠草原南部边缘地带盐碱滩地中捕获的田鼠标本,经形态鉴定及DNA条形码比对确定为东方田鼠。首次在陕西省定边县鼠疫自然疫源地记录东方田鼠,为进一步在该地区进行病媒、鼠疫及相关疾病监测提供了基础依据。

关键词: 鼠种鉴定, 鼠疫疫源地, 东方田鼠

Abstract: Objective To identify and classify newly-recorded rodent species in the epidemic focus of plaque in Dingbian county, Shaanxi province. Methods Morphological observation and DNA barcode technology were applied for rodent identification and classification. Results A total of 6 voles were captured with the body length between 120 mm and 140 mm, body weight ranging from 41.75 g to 71.58 g and skull length less than 32 mm. The tail length exceeded one third of the body length. The voles were covered by dark brown skin but their abdomen was sand-yellow in colour. The COⅠgene extracted from the samples was amplified through PCR and sequenced, with the maximum genetic distance between these samples and those of Microtus fortis from other areas being 1%. Conclusion The voles captured from the salt marshes in the southern edge of Ordos Plateau in the plague area of Dingbian county are identified as M. fortis through morphological analysis and DNA comparision. M. fortis is recorded for the first time in the plague natural foci of Shaanxi province, which provides useful information and a basis for further monitoring of vetor, plaque and related diseases in the area.

Key words: Identification, Plague focus, Microtus fortis

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