中国媒介生物学及控制杂志 ›› 2012, Vol. 23 ›› Issue (3): 255-258.

• 调查研究 • 上一篇    下一篇

义乌市2001-2010年鼠疫历史疫区宿主和媒介调查

骆淑英, 傅涛, 朱志宏   

  1. 义乌市疾病预防控制中心防病一科, 浙江义乌 322000
  • 收稿日期:2012-03-05 出版日期:2012-06-20 发布日期:2012-06-20

Investigation on plaque hosts and vectors in the areas with a history of the epedemic in Yiwu city, 2001 to 2010

LUO Shu-ying, FU Tao, ZHU Zhi-hong   

  1. Yiwu Center for Disease Control and Prevention, Yiwu 322000, Zhejiang Province, China
  • Received:2012-03-05 Online:2012-06-20 Published:2012-06-20

摘要: 目的 调查2001-2010年义乌市鼠疫历史疫区宿主和媒介情况,为义乌市鼠疫预防控制提供科学依据。方法 采用笼捕法,在义乌市监测点捕获鼠类,乙醚麻醉后收集蚤类,取鼠肝、脾进行分离培养或PCR检测,采集鼠血清用放射免疫试验或间接血凝试验进行血清学检测,分别计算鼠捕获率、染蚤率、蚤指数等,并用SPSS 16.0软件对数据进行统计分析。结果 2001-2010年义乌市鼠平均捕获率室内为1.06%,野外为1.23%;家鼠以褐家鼠和黄胸鼠为主,野鼠以黑腹绒鼠和黑线姬鼠为主;鼠类平均染蚤率为1.52%,平均蚤指数为0.032;分离培养7012份鼠标本和169份蚤类标本,均为阴性;血清学监测6884份标本,4份阳性。结论 义乌市鼠疫宿主和媒介构成稳定,密度较低,但应加强对血清学阳性的研究,提高对输入病例的警惕性。

关键词: 鼠疫, 宿主, 媒介, 调查

Abstract: Objective To get epidemiological data on plaque hosts and vectors in the areas with a history of the epidemic in Yiwu city, providing a scientific basis for the prevention and control of the epidemic in the city. Methods Rodents were captured in the surveillance sites in the city using live traps, and fleas on them were collected under ether anaesthesia. Rodent liver and spleen were taken for isolation and culture or for PCR detection. Radio immunoassay (RIA) or indirect hemagglutination assay (IHA) was used for serological study. Indices such as rodent capture rate, infection rate and flea index were calculated. SPSS 16.0 software was applied for statistical analysis of the data. Results The average indoor and outdoor rodent capture rate was 1.06% and 1.23%, respectively. The primary indoor rodents were Rattus norvegicus and R. tanezumi, while Eothenomys melanogaster and Apodemus agrarius were the most common outdoor rodents. The flea infection rate was 1.52% with an average flea index of 0.032. No plague were cultured or detected in 7012 rodent samples and 169 flea samples. Plague antibody was detected in 4 out of 6884 samples. Conclusion The composition of plaque hosts and vectors is stable with a low density of rodents and fleas in Yiwu city.However, serological study should be strengthened to be vigilant against imported cases.

Key words: Plague, Host, Vector, Investigation

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