德国小蠊钠离子通道抗性研究进展

摘要/Abstract

摘要：

德国小蠊在医学与经济方面的重要性和危害性促使人们对其进行广泛的化学防治,但随之产生严重的抗性。该文以德国小蠊钠离子通道抗性即击倒抗性(knockdown resistance,kdr)为主题,重点论述了德国小蠊kdr 抗性的发现历史、分子与进化机制,扼要介绍了钠离子通道结构特征、kdr 突变的功能研究方法以及近期所取得的代表性研究结果。此外,概述了该领域的最新研究方向及结果对德国小蠊防制、抗性监测与治理等的理论与指导意义。

关键词: 德国小蠊, 击倒抗性, 钠离子通道, 点突变

Abstract:

Wide application of insecticides has resulted in severe resistance in the German cockroach (Blattella germanica). This study reviews the discovery and molecular and evolutional mechanisms of the knockdown resistance (kdr), also known as the sodium channel resistance, with emphasis on the molecular structure, functional research Methods of kdr mutations and up-to- date representative results. In addition, the latest findings are summarized to inform the prevention, control and resistance monitoring of the cockroaches.

Key words: Blattella germanica, Sodium channel, Knockdown resistance, Point mutation

中图分类号:

R384.9

周小洁, 钱坤, 曾晓芃. 德国小蠊钠离子通道抗性研究进展[J]. 中国媒介生物学及控制杂志, 2011, 22(6): 611-614.

ZHOU Xiao-jie, QIAN Kun, ZENG Xiao-peng. Review of sodium channel resistance in the Blattella germanica[J]. Chines Journal of Vector Biology and Control, 2011, 22(6): 611-614.

参考文献

[1] Heal RE,Nash KB,Williams M. An insecticide resistance strain ofthe German cockroach from Corpus Christi［J］. Texas J EconEntomol,1953,46:385-386.

[2] Soderlund DM,Knipple DC. The molecular biology of knockdownresistance to pyrethroid insecticides［J］. Insect Biochem Mol Biol, 2003,33(6):563-577.

[3] 曾晓芃. 德国小蠊防制技术及抗性治理对策研究进展［J］. 中华卫生杀虫药械,2005,11(4):220-224.

[4] Busvine JR. Mechanism of resistance to insecticide in houseflies［J］. Nature,1951,168(4266):193-195.

[5] Sawicki RM. Unusual response of DDT?resistant houseflies tocarbinol analogues of DDT［J］. Nature,1978,275(5679):443-444.

[6] Soderlund DM,Knipple DC. Knockdown resistance to DDT andpyrethroids in the house fly(Diptera:Muscidae):from genetic traitto molecular mechanism［J］. Ann Ent Soc Amer,1999,92:909-915.

[7] Scott JG,Matsumura F. Characteristics of a DDTinduced case ofcross - resistance to permethrin in Blattella germanica［J］. PesticBiochem Physiol,1981,16:21-27.

[8] Loughney K,Krebe R,Ganetzk B. Molecular analysis of the Paralocus,a sodium channel gene in Drosophila［J］. Cell,1989,58(6):1143-1154.

[9] Knipple DC,Payne LL,Soderlund DM. PCR?generated conspecificsodium channel gene probe for the house fly［J］. Arch InsectBiochem Physiol,1991,16(1):45-53.

[10] Williamson MS,Denholm I,Bell CA,et al. Knockdown resistance(kdr)to DDT and pyrethroid insecticides maps to a sodium channelgene locus in the housefly(Musca domestica)［J］. Mol Gen Genet,1993,240(1):17-22.

[11] Knipple DC,Doyle KE,Marsella?Herrick PA,et al. Tight geneticlinkage between the kdr insecticide resistance trait and a voltage?sensitive sodium channel gene in the house fly［J］. Proc Natl AcadSci USA,1994,91(7):2483-2487.

[12] Dong K,Scott JG. Linkage of kdr?type resistance and the Para?homologous sodium channel gene in German cockroaches(Blattellagermanica)［J］. Insect Biochem Mol Biol,1994,24(7):647-654.

[13] Miyazaki M, Ohyama K, Dunlap DY, et al. Cloning andsequencing of the Para?type sodium channel gene from susceptibleand kdr ?resistant German cockroaches(Blattella germanica)andhouse fly(Musca domestica)［J］. Mol Gen Genet,1996,252(1-2):61-68.

[14] Dong K. A single amino acid change in the Para sodium channelprotein is associated with knockdown?resistance(kdr)to pyrethroidinsecticides in the German cockroach［J］. Insect Biochem Mol Biol,1997,27(2):93-100.

[15] Dong K,Valles SM,Scharf ME,et al. The knockdown resistance(kdr) mutation in pyrethroid ? resistant German cockroaches［J］.Pestic Bioche Phys,1998,60(3):195-204.

[16] Liu Z,Valles SM,Dong K. Novel point mutations in the Germancockroach Para sodium channel gene are associated withknockdown resistance(kdr) to pyrethroid insecticides［J］. InsectBiochem Mol Biol,2000,30(10):991-997.

[17] Tan J,Liu Z,Tsai TD,et al. Novel sodium channel gene mutationsin Blattella germanica reduce the sensitivity of expressed channelsto deltamethrin［J］. Insect Biochem Mol Biol,2002,32(4):445-454.

[18] Lee SH,Smith TJ,Knipple DC,et al. Mutations in the house flyVssc1 sodium channel gene associated with super ? kdr resistanceabolish the pyrethroid sensitivity of Vssc1/tipE sodium channelsexpressed in Xenopus oocytes［J］. Insect Biochem Mol Biol,1999,29(2):185-194.

[19] Feng G,Deák P,Chopra M,et al. Cloning and functional analysisof tipE,a novel membrane protein that enhances Drosophila Parasodium channel function［J］. Cell,1995,82(6):1001-1011.

[20] Lee SH,Smith TJ,Ingles PJ,et al. Cloning and functionalcharacterization of a putative sodium channel auxiliary subunit genefrom the house fly(Musca domestica)［J］. Insect Biochem Mol Biol,2000,30(6):479-487.

[21] Warmke JW,Reenan RA,Wang P,et al. Functional expression ofDrosophila Para sodium channels:modulation by the membraneprotein tipE and toxin pharmacology［J］. J Gen Physiol,1997,110(2):119-133.

[22] Liu Z,Tan J,Valles SM,et al. Synergistic interaction between twocockroach sodium channel mutation and a tobacco budworm sodiumchannel mutation in reducing channel sensitivity to a pyrethroidinsecticide［J］. Insect Biochem Mol Biol,2002,32(4):397-404.

[23] Du Y,Liu Z,Nomura Y,et al. An alanine in segment 3 of domainⅢ(ⅢS3)of the cockroach sodium channel contributes to the lowpyrethroid sensitivity of an alternative splice variant［J］. InsectBiochem Mol Biol,2006,36(2):161-168.

[24] Du Y,Nomura Y,Luo N,et al. Molecular determinants on the insectsodium channel for the specific action of type Ⅱ pyrethroidinsecticides［J］. Toxicol Appl Pharmacol,2009,234(2):266-272.

[25] Du Y,Garden D,Wang L,et al. Identification of new batrachotoxin?sensing residues in segment Ⅲ S6 of sodium channel［J］. J BiolChem,2011,doi:10.1074/jbc.M110.208496.

[26] Hu Z,Du Y,Nomura Y,et al. A sodium channel mutationidentified in Aedes aegypti selectively reduces cockroach sodiumchannel sensitivity to typeⅠ,but not typeⅡ pyrethroids［J］. InsectBiochem Mol Biol,2011,41(1):9-13.

[27] Rinkevich FD,Zhang L,Hamm RL,et al. Frequencies of thepyrethroid resistance alleles of Vssc1 and CYP6D1 in house fliesfrom the eastern United States［J］. Insect Mol Biol,2006,15(2):157-167.

[28] Valles SM,Perera OP,Strong CA. Relationship between the Para?homologous sodium channel point mutation(G→C at nucleotide2979)and knockdown resistance in the German cockroach usingmultiplex polymerase chain reaction to discern genotype［J］. J EconEntomol,2003,96(3):885-991.

[29] 张晓龙. 德国小蠊对氯菊酯击倒抗性与基因频率关系的研究
［D］. 北京:中国人民解放军军事医学科学院,2004.

[30] Liu N,Xu Q,Zhang L. Sodium channel gene expression inmosquitoes,Aedes albopictus(S.)［J］. Insect Sci,2006,13(6):431-436.

[31] Xu Q,Wang H,Zhang L,et al. Sodium channel gene expressionassociated with pyrethroid resistant house flies and Germancockroaches［J］. Gene,2006,379:62-67.

[32] Xu Q,Wang H,Zhang L,et al. Kdr allelic variation in pyrethroidresistant mosquitoes, Culex quinquefasciatus(S.)［J］. BiochemBiophys Res Commun,2006,345(2):774-780.

[33] Donnelly MJ,Corbel V,Weetman D,et al. Does kdr genotypepredict insecticide?resistance phenotype in mosquitoes［J］. TrendsParasitol,2009,225(5):213-219.