关键词: 家蝇幼虫, 血淋巴, 蛋白, 人宫颈癌Hela细胞株, 细胞凋亡

Abstract:

Objective To observe the morphological changes of Hela human cervical carcinoma cells in vitro after treatment with the anticancer protein from Musca domestica larva hemolymph(MAC-1) protein derived from the immuned hemolymph of housefly larvae. Methods The growth inhibition rates and cytotoxic effects of Hela cells were measured with MTT assay. The treated cells were observed under inverted microscope and morphological changes were tested with HE and microscope. The ultrastructure changes were observed under transmission electron microscope. Results The MAC-1 protein could significantly inhibit the growth and proliferation of Hela cells in a dose- and time- dependent manner. Under inverted microscope, the Hela cells showed the apoptosis after treated with 12.5 μg/ml of the MAC-1 in 12 hours. The cells showed classic morphological features of apoptosis under light microscope and electronic microscope. Conclusion The MAC-1 protein inhibited proliferation and induced apoptosis of Hela cells in vitro.

Key words: Musca domestica larvae, Hemolymph, Protein, Hela human cervical carcinoma cell, Apoptosis