云南省东北等地区蚊虫及蚊媒病毒调查研究

中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (4): 304-308,312.

云南省东北等地区蚊虫及蚊媒病毒调查研究

杨杜鹃¹, 付士红², 张海林³, 杨卫红³, 冯云³, 王静林³, 章域震³, 王丕玉⁴, 陈维欣², 梁国栋²

1 大理学院公共卫生学院,云南大理 671000;
2 中国疾病预防控制中心病毒病预防控制所病毒性脑炎室,北京 102206;
3 云南省地方病防治所/云南省病毒立克次体研究中心,大理 671000;
4 云南省寄生虫病防治所

收稿日期:2010-12-29 出版日期:2011-08-20 发布日期:2011-08-20
通讯作者: 梁国栋,Email: gdliang@hotmail.com; 张海林,Email: zhangHL715@163.com
作者简介:杨杜鹃(1983-),女,在读硕士,从事分子流行病学研究。Email: yangdujuan.2008@163.com;付士红(1967-),女,主管技师,主要从事虫媒病毒的相关研究。Email: shihongfu@hotmail.com
基金资助:
中美传染病合作项目(U19-GH000004);中日健康基金(2002-2007);国家自然科学基金(30560142)

Investigation of mosquitoes and mosquito-borne viruses in northeast Yunnan province

YANG Du-juan¹, FU Shi-hong², ZHANG Hai-lin³, YANG Wei-hong³, FENG Yun³, WANG Jing-lin³, ZHANG Yu-zhen³, WANG Pi-yu⁴, CHEN Wei-xin², LIANG Guo-dong²

1 School of Public Health,Dali University, Dali 671000, Yunnan Province, China;
2 Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
3 Yunnan Institute of Endemic Disease Control and Prevention/ Research Center of Arbovirus and Rickettsia,Dali 671000, Yunnan Province, China;
4 Yunnan Institute of Parasitic Disease

Received:2010-12-29 Online:2011-08-20 Published:2011-08-20
Supported by:
Supported by the China-US Collaborative Program on Emerging and Re-Emerging Infectious Diseases(No. U19-GH000004), the Japan Health Science Foundation(No. 2002-2007),and National Natural Science Foundation of China(No. 30560142)

摘要/Abstract

摘要：

目的了解滇东北等地区蚊虫及蚊传虫媒病毒分布特点,为虫媒病毒病防治提供科学依据。方法 2009年在滇东北等地区的6个县采集蚊虫标本;蚊虫经分类鉴定后,用细胞培养法分离病毒,对病毒分离物进行分子生物学鉴定。结果共采集到4属(库蚊、按蚊、阿蚊、伊蚊)24种18 562只蚊虫,其中三带喙库蚊、中华按蚊为主要蚊种,构成比分别为58.37%和28.45%;从蚊虫标本中分离到15株病毒,经分子生物学鉴定,其中2株(YN0911和YN0967)为基因Ⅰ型流行性乙型脑炎(乙脑)病毒,均分离自三带喙库蚊;1株(YN0922)为版纳病毒,分离自中华按蚊;12株为淡色库蚊浓核病毒,其中9株分离自三带喙库蚊,3株分离自中华按蚊。结论三带喙库蚊和中华按蚊为调查地区的优势蚊种,并携带乙脑病毒、版纳病毒和淡色库蚊浓核病毒;滇东北地区首次分离到乙脑病毒。

关键词: 蚊科, 虫媒病毒, 脑炎病毒, 日本, 版纳病毒, 浓核病毒亚科

Abstract:

Objective To investigate the distribution patterns of mosquitoes and mosquito-borne viruses in Yunnan province to provide evidence for prevention and control of mosquito-borne diseases. Methods In 2009, mosquito samples were collected from 6 counties of Yunnan province. Mosquitoes were first classified, followed by cell culturing for viral isolation for molecular identification. Positive isolates were identified using SDS-PAGE and RT-PCR. The genomes of isolates were also, sequenced for phylogenetic analysis. Results A total of 18 562 mosquitos of 4 genera (Culex, Anopheles, Armigeres, Aedes) and 24 species were collected. Cx. tritaeniorhynchus and An. sinensis were the major species, comprising 58.37% and 28.45% of the total population, respectively. Fifteen strains of viruses were isolated from the mosquito pools. RT-PCR and phylogenetic analysis revealed two viral strains from Cx. tritaeniorhynchus, identified as Japanese encephalitis virus (JEV, GenotypeⅠ). One strain isolated from An. sinensis was identified as Banna virus. Twelve vrial strains were identified as Cx. pipiens pallens densovirus (CppDNV), of these, nine strains were isolated from Cx. tritaeniorhynchus, and three strains from An. sinensis. Conclusion Cx. tritaeniorhynchus and An. sinensis were the predominant species in the investigated areas. JEV, Banna virus and CppDNV were isolated. In northeast Yunnan, JEV was isolated for the first time.

Key words: Culicidae, Arboviruses, Encephalitis virus, Japanese, Banna virus, Densovirinae

中图分类号:

杨杜鹃, 付士红, 张海林, 杨卫红, 冯云, 王静林, 章域震, 王丕玉, 陈维欣, 梁国栋. 云南省东北等地区蚊虫及蚊媒病毒调查研究[J]. 中国媒介生物学及控制杂志, 2011, 22(4): 304-308,312.

YANG Du-juan, FU Shi-hong, ZHANG Hai-lin, YANG Wei-hong, FENG Yun, WANG Jing-lin, ZHANG Yu-zhen, WANG Pi-yu, CHEN Wei-xin, LIANG Guo-dong. Investigation of mosquitoes and mosquito-borne viruses in northeast Yunnan province[J]. Chines Journal of Vector Biology and Control, 2011, 22(4): 304-308,312.

参考文献

[1] 张海林. 云南省虫媒病毒研究进展
[J]. 中国媒介生物学及控制杂志,2004,15(5):410-414.

[2] 冯云,张海林,梁国栋. 我国从节肢动物中新分离的虫媒病毒
[J].中国媒介生物学及控制杂志,2009,20(2):178-181.

[3] 张海林,陶三菊,张云智. 云南省澜沧江下游地区虫媒病毒研究状况
[J]. 中华实验和临床病毒学杂志,2006,20(1):95-98.

[4] 张海林,陶三菊,杨冬荣,等. 云南首次分离到辛德毕斯(Sindbis)、巴泰(Batai)和Colti 病毒
[J]. 中国人兽共患病杂志,2005,21(7):548-551,557.

[5] 周涛,张海林,李铭华,等. 云南省文山中越边境地区虫媒病毒调查
[J]. 中华预防医学杂志,2009,43(12):1086-1090.

[6] 孙肖红,张晓龙,刘宇夫,等. 云南省1株版纳病毒的分离和鉴定
[J]. 中华流行病学杂志,2010,31(6):685-687.

[7] 张云智,张海林,米竹青,等. 云南省河口市蚊虫及虫媒病毒调查
[J]. 医学动物防制,1998,14(5):37-38.

[8] 张海林,自登云,龚正达,等. 云南省孟连县蚊虫调查及乙型脑炎病毒分离
[J]. 医学动物防制,1998,14(2):9-11.

[9] 张海林,米竹青,张云智,等. 云南省边境地区蚊虫自然感染乙型脑炎病毒的研究
[J]. 中国媒介生物学及控制杂志,2002,13(2):101-104.

[10] Uchil,PD,Satchidanandam V. Phylogenetic analysis of Japaneseencephalitis virus envelope gene based analysis reveals a fifthgenotype,geographic clustering,and multiple introductions of thevirus into the Indian Subcontinent
[J]. Am J Trop Med Hyg,2001,65(3):242-251.

[11] Solomon T,Ni H,Beasley DWC,et al. Origin and evolution ofJapanese encephalitis virus in Southeast Asia
[J]. J Virol,2003,77(5):3091-3098.

[12] Williams DT,Wang LF,Daniels PW,et al. Molecular characterizationof the first Australian isolate of Japanese encephalitis virus,the FUstrain
[J]. J Gen Virol,2000,81(Pt 10):2471-2480.

[13] Nga PT, Parquet MC, Cuong VD, et al. Shift in Japaneseencephalitis virus(JEV)genotype circulating in Northern Vietnam:implications for frequent introductions of JEV from Southeast Asiato East Asia
[J]. J Gen Virol,2004,85(Pt 6):1625-1631.

[14] 孙肖红,付士红,张海林,等. 云南省虫媒病毒的分离鉴定
[J]. 中华实验和临床病毒学杂志,2005,19(4):319-324.

[15] Wang JL,Pan XL,Zhang HL,et al. Japanese encephalitis virusesfrom bats in Yunnan,China
[J]. Emerg Infect Dis,2009,15(6):939-942.

[16] 徐丽宏,梁国栋. Coltivirus 病毒属研究进展
[J]. 中国病毒学,2002,17(4):385-391.

[17] Liu H,Li MH,Zhai YG,et al. Banna virus,China,1987-2007
[J]. Emerg Infect Dis,2010,16(3):514-517.

[18] Zhai YG,Lv XJ,Sun XH,et al. Isolation and characterization ofthe full coding sequence of a novel densovirus from the mosquitoCulex pipiens pallens
[J]. J Gen Virol,2008,89(Pt 1):195-199.

[19] 吕新军,吕志,孙肖红,等. 新疆南部地区蚊虫中病毒分离和初步鉴定
[J]. 中华预防医学杂志,2009,43(5):395-398.