埃及伊蚊传播西尼罗病毒的媒介效能研究

中国媒介生物学及控制杂志 ›› 2011, Vol. 22 ›› Issue (2): 107-109.

埃及伊蚊传播西尼罗病毒的媒介效能研究

张映梅¹, 姜淑芳^1,2, 郭晓霞¹, 董言德¹, 赵彤言¹

1 军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室，北京 100071;
2 中国人民解放军总医院

收稿日期:2010-10-22 出版日期:2011-04-20 发布日期:2011-04-20
通讯作者: 赵彤言，Email: zhaoty@nic.bmi.ac.cn
作者简介:张映梅(1979-)，女，博士，助理研究员，从事媒介生物学和防治研究。Email: zymmosquito@126.com
基金资助:
国家自然科学基金 (30371256); 国家科技部攻关项目(2003BA712A09202)

Experimental studies on the potential of Aedes aegypti to transmit West Nile virus in China

ZHANG Ying-mei¹, JIANG Shu-fang^1,2, GUO Xiao-xia¹, DONG Yan-de¹, ZHAO Tong-yan¹

1 Academy of Military Medical Science, State Key Laboratory of Pathogen and Biosecurity Beijing 100071, China;
2 Chinese People’s Liberation Army General Hospital

Received:2010-10-22 Online:2011-04-20 Published:2011-04-20
Supported by:
Supported by the National Natural Science Foundation of China (No. 30371256) and Chinese National Programs for Science and Technology Development (No. 2003BA712A09202)

摘要/Abstract

摘要：

目的研究我国埃及伊蚊传播西尼罗病毒的潜在能力。方法人工配制病毒血餐感染埃及伊蚊并进行传播实验，应用反转录聚合酶链反应(RT-PCR)、病毒分离和间接免疫荧光法(IFA)检测蚊虫和来亨鸡体内病毒。结果动物实验结果表明敏感动物来亨鸡感染西尼罗病毒后，第2天其体内病毒量达到最高，说明进行传播实验后的第2天是检测传播是否成功的最佳时间。感染与传播实验结果表明埃及伊蚊对西尼罗病毒的感染率达到了63.3%，而且通过刺叮敏感动物(来亨鸡)能够传播该病毒，其传播率为30.0%。结论在未来我国可能的西尼罗热暴发中，埃及伊蚊可能成为其在海南等地的潜在传播媒介。

关键词: 埃及伊蚊, 西尼罗病毒, 易感性, 传播

Abstract:

Objective To evaluate the potential of Aedes aegypti to transmit West Nile virus(WNV) in China. Methods Ae. aegypti was infected orally with WNV and after 12 to 14 days’ incubation allowed to transmit the virus through bite wounds. RT-PCR, virus isolation with C6/36 and indirect immunofluorescence assay were implimented to detect WNV in experimentally infected mosquitoes and Leghorn chicken. Results It was found that the WNV loaded in the susceptible host leghorn was the highest in the 2nd day after infection, indicating that it was the best time for the evaluation of the transmission. Ae. aegypti could be infected by WNV with a susceptibility rate of 63.3%, and transmitted the virus to healthy Leghorn chicken by bite wounds with a transmission rate is 30.0%. Conclusion Ae. aegypti is a potential vector of WNV in some areas such as Hainan province, likely to cause outbreaks of West Nile fever in China in future. Actions are to be taken for the control and prevention of the vecor.

Key words: Aedes aegypti, West Nile virus, Susceptibility, Transmission

中图分类号:

张映梅, 姜淑芳, 郭晓霞, 董言德, 赵彤言. 埃及伊蚊传播西尼罗病毒的媒介效能研究[J]. 中国媒介生物学及控制杂志, 2011, 22(2): 107-109.

ZHANG Ying-mei, JIANG Shu-fang, GUO Xiao-xia, DONG Yan-de, ZHAO Tong-yan. Experimental studies on the potential of Aedes aegypti to transmit West Nile virus in China[J]. Chines Journal of Vector Biology and Control, 2011, 22(2): 107-109.

参考文献

[1] Campbell GL， Marfin AA， Lanciotti RS，et al. West Nile virus
[J].Lancet Infect Dis，2002，2(9)：519-529.

[2] Zeller HG， Schuffenecker I. West Nile virus： An overview of its spread in Europe and the Mediterranean Basin in contrast to its spread in the American
[J]. Eur J Clin Microbiol Infect Dis，2004， 23(3)：147-156.

[3] 陈伯权，刘琴芝，周国芳. 我国一些地区人血清的虫媒病毒抗体调查
[J]. 中华流行病学杂志，1983，4(5)：263-266.

[4] 姜淑芳，赵彤言，董言德，等. 实验感染的三带喙库蚊和来亨鸡体内西尼罗病毒的分离与鉴定
[J]. 昆虫学报，2006，49(4)：588-592.

[5] Goddard LB，Roth AE，Reisen WK. Vector competence of California mosquitoes for West Nile virus
[J]. Emerg Infect Dis，2002，8(12)：1385-1391.

[6] 郭晓霞，赵彤言，董言德，等. 登革Ⅱ型病毒经白纹伊蚊滞育卵的传递
[J]. 昆虫学报，2004，47(4)：424-428.

[7] Kauffman EB， Jones SA， Dupuis AP 2nd， et al. Virus detection protocols for West Nile virus in vertebrate and mosquito specimens
[J]. J Clin Microbiol，2003，41(8)：3661-3667.

[8] Shi PY， Kauffman EB， Ren P， et al. High-throughput detection of West Nile virus RNA
[J]. J Clin Microbiol，2001，39(4)：1264-1271.

[9] 姜淑芳，赵彤言，张映梅，等. 实验感染蚊虫及来亨鸡体内西尼罗病毒的RT-PCR检测
[J]. 寄生虫与医学昆虫学报，2006，13(1)：21-24.

[10] Turell MJ， Oguinn ML， Dohm DJ， et al. Vector competence of North American mosquitoes(Diptera：Culicidae) for West Nile virus
[J]. J Med Entomol，2001，38(2)：130-134.

[11] Turell MJ，Dohm DJ，Sardelis MR， et al. An update on the potential of north American mosquitoes(Diptera：Culicidae) to transmit West Nile virus
[J]. J Med Entomol，2005，42(12)：57-62.

[12] Work TH， Hurlbut HS， Taylor RM. Indigenous wild birds of the West Nile Delta as potential West Nile virus circulating reservoirs
[J]. Am J Trop Med Hyg，1955，4(5)：872-888.

[13] Hayes GG， Basit A， Bagar S， et al. Vector competence of Culex tritaeniorhynchus (Diptera：Culicidae) for West Nile virus
[J]. J Med Entomol，1980，17(2)：172-177.

[14] Gubler DJ, Rosen L. Variation among geographic strains of Aedes albopictus in susceptibility to infection with dengue viruses
[J]. Am J Trop Med Hyg，1976，25(2)：318-325.

[15] Hardy JL， Reeves WC， Sjogren RD. Variation in the susceptibility of field and laboratory populations of Culex tarsalis to experimental infection with western equine encephalomyelitis virus
[J]. Am J Epidemiol，1976，103(5)：498-505.

[16] Sardelis MR，Turell MJ，O’Guinn ML，et al. Vector competence of three North American strains of Aedes albopictus for West Nile virus
[J]. J Am Mosq Control Assoc，2002，18(4)：284-289.