关键词: 家蝇, 乙酰胆碱酯酶, 抗药性, 残杀威

Abstract:

Objective Propoxur-susceptible Musca domestica strains were established through reverse selective breeding in the laboratory setting. The discriminating concentration of insensitive acetylcholinesterase (AChE) was established according to the AChE sensitivity of the strains. The insensitive gene AChE phenotypes of outdoor M. domestica populations were investigated to provide the basis for delaying pesticide resistance development in M. domestica. Methods The susceptible strains were bred through elimination selection. The insensitive AChE phenotypes in the natural population were investigated by biochemical approaches. Results After 6 cycles of elimination, the sensitivity of the original susceptible strain of M. domestica to propoxur had a 13.87 fold increase. The investigation result showed that more than 40% of the natural population in Beijing had insensitive AChE. Conclusion Carrying insensitive AChE, the susceptible strain of M. domestica should be subject to further reverse selective breeding. The natural population was resistant to propoxur. Hence, the application of this pesticide should be stopped and replaced with an alternative to avoid or delay the development of pesticide resistance.

Key words: Musca domestica, Acetylcholinesterase, Insecticide resistance, Propoxur