关键词: 家蝇, 抗药性, 半数致死剂量, 方差分析

Abstract:

Objective To determine the dynamics of the resistance of outdoor natural populations of Musca domestica to six insecticides including deltamethrin in seven cities such as Ningbo, providing the basis for scientific and proper use of pesticides and further development of fly control. Methods Topical application was used to determine the median lethal dose of the agents to M. domestica. Results The natural populations of M. domestica in the seven cities were most resistant to deltamethrin and beta cypermethrin, the resistance ratios for which were: 34.9 and 22.6 times in Ningbo, 38.6 and 20.8 times in Lishui, 36.4 and 23.5 times in Wenzhou, 28.3 and 30.5 times in Quzhou, 40.1 and 36.1 times in Jinhua, 34.1 and 32.0 in Shaoxing, and 37.9 and 20.4 times in Linhai. The resistance ratios for dichlorvos and cypermethrin, to which the flies were less resistant, were: 10.3 and 15.7 times in Ningbo, 10.9 and 12.5 times in Lishui, 11.2 and 17.5 times in Wenzhou, 13.8 and 12.6 times in Quzhou, 14.3 and 11.2 times in Jinhua, 10.7 and 16.3 times in Shaoxing, and 15.2 and 10.9 times in Linhai. The resistance ratios of the flies in Quzhou and Jinhua to permethrin were 10.1 and 13.0 times, respectively, while all of those in the other five cities were below 8.2 times. The resistance ratio of all the flies to while acetophenate was below 1.9 times. Analysis of variance showed significant difference in the resistance between the natural populations of M. domestica in the seven cities and the susceptible lines (F＝ 51.299, P＝0.000), though no statistical difference was found in the resistance between the natural populations from different regions (F＝0.100, P＝0.998). Analysis of variance showed statistical difference in the varied insecticidal resistance of the natural populations to different agents (F＝25.420, P＝0.000). Conclusion The natural populations demonstrated varied resistance to the 6 pesticides, and were most resistant to deltamethrin and beta cypermethrin.

Key words: Musca domestica, Insecticide resistance, Median lethal dose, Analysis of variance