关键词: 家蝇, 溴氰菊酯, 抗药性, 聚合酶链反应

Abstract:

Objective　The study was conducted to analyze the molecular mechanisms of deltamethrin resistance in house flies (Musca domestica). Methods　The deltamethrin?resistant strains of housefly were analyzed using primers properly designed based on the conservative sequence of P450 gene CYP6D1 in the laboratory. Specific fragments were amplified in both sensitive and resistant strains by PCR. Results　A specific fragment of 210 bp was obtained from both sensitive and resistant strains, demonstrating no difference in the fragment size and band brightness between the two. It was thus indicated that no noticeable mutation was present in P450 gene in resistant house flies at the DNA level. RT?PCR results revealed that amplified bands in resistant strains were brighter than those in sensitive ones, suggesting a different volume of RNA as the reverse transcription activity of P450 gene might be enhanced in resistant strains. Conclusion　Deltamethrin resistance in house flies was probably correlated with the elevated reverse transcription activity of P450 in the resistant strains.

Key words: Musca domestica, Deltamethrin, Insecticides resistance, Polymerase chain reaction