关键词: 中华按蚊, 产卵反应, 产卵器, 负趋性, 寄主细胞, 成对t测验

Abstract:

Objective　To determine the oviposition responses of Anopheles sinensis to bacteria A4 (unidentified), Aeromonas sp. and Enterobacter sp. and whether or not the three strains attracted the mosquitoes, providing theoretical and experimental evidence for the application of the three bacteria in the intervention of malaria. Methods　Blood?fed and held for 48-60 h, copulated female An. sinensis were placed into cages with filter?paper oviposition cups that contained different bacteria in darkness for 12 h egg laying. The numbers of eggs in the oviposition cups counted, the difference was analyzed using paired t?test. Results　Gravid An. sinensis laid the most eggs in the oviposition cups containing Enterobacter sp. The numbers in those containing bacteria A4 and Aeromonas sp. were significantly lower than those in the control cups and those containing Enterobacter sp., with the P values of 0.026, 0.012, 0.048 and 0.016, respectively. Despite slightly greater number of eggs in the oviposition cups of Enterobacter sp. compared to the control group, there was no noticeable difference between the two (P＞0.05). Conclusion　Gravid An. sinensis demonstrated significant negative taxis towards bacteria A4 and Aeromonas sp., as well as slight taxis to Enterobacter sp., implying that the three bacteria were not the dietary preference for An. sinensis. However, in light of the possibility of their stable colonization into mosquito’s guts, these strains may serve as the host cells of larvicide genes and transgenic anopheline antimalarial genes. 

Key words: Anopheles sinensis, Oviposition response, Oviposition cups, Negative taxis, Host cells, Paired t?test