关键词: 鼠疫, 胶体金免疫实验, 流行病学调查

Abstract: Objective To study the sensitivity of colloidal gold immunobinding and analyze the distribution of crowd bubonic plague F1 immune body in Hebei province.Methods 196 serums and 162 serums of healthy people were sampled from history areas affected by plague and plague foci among animals(Zhangjiakou city) in Hebei natural plague foci and fur processing workers in Baoding.100 serums were collected from non-plague foci as control.Colloedal gold immunobinding and indirect hemagglutination test(IHA) were used to detect,at the same time,the minimum limit of detection of these two methods was compared.Results There were 3 positive serums in 196 serum samples,and the positive rate was 1.53%.None positive samples was found in 162 serum samples.The control were all negative.Conclusion The minimum detection of colloedal gold immunobinding was less than that of IHA.There were positive serum samples in plague natural foci,and the people age whose serum presented positive exceeded 40 years old.The samples collected from Baoding fur processing workers and non-plague foci were all negative.2 samples were positive after vaccination by investigation,and others needed be further explored.