中国媒介生物学及控制杂志

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青藏铁路沿线鼠疫菌外膜蛋白种类的研究

丛显斌;张春华;赵斌;吕景生;王忠惠;张市;邵奎东   

  1. 中国疾病预防控制中心鼠疫布鲁氏菌病预防控制基地 白城137000
  • 出版日期:2008-10-20 发布日期:2008-10-20

A study on the membrane proteins varieties of the Yersinia pestis along the Qinghai Tibet raiway

CONG Xian-bin; ZHANG Chun-hua; ZHAO Bin; LV Jing-sheng; WANG Zhong-hui; ZHANG Shi; SHAO Kui-dong   

  1. The Base for Control and Prevention of Plague and Brucellosis, Chinese Center for Disease Control and Prevention, Baicheng 137000, China
  • Online:2008-10-20 Published:2008-10-20

摘要: 目的 研究并分析青藏铁路没线鼠疫菌外膜蛋白种类及相对分子质量。方法 鼠疫菌经破碎后采取超高速离心法提取外膜蛋白,利用十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE)系统分离鼠疫菌外膜蛋白。结果 在10%的SDS-PAGEH上,37℃条件下的培养物可表达26条件外膜蛋白。结论 试验用鼠疫菌株可分泌标准的外膜蛋白,属YOP1型。

关键词: 青藏铁路沿线, 鼠疫菌, 外膜蛋白

Abstract: Objective To study and analyse the molecular weight and outer membrane proteins varieties of the Yersina pestis.Methods The outer membrane protein was extracted by ultra-centrifuge after the Y.pestis fragmentation and islated by SDS-PAGE rlrvytophotrdid. Results There were 26stripes of outer membrance proteins at 37℃ and 36stripes at 28℃ in 10% SDS-PAGE electorphoresis. Conclusion The Y.pestis strains contained standard outer membrand proteins,which belonged to YOP1.