关键词: 钉螺, 内生真菌, 杀螺作用, 金钱松, JJ18, ITS序列

Abstract: Objective To study the molluscicidal effect of endophyte JJ18 from Pseudolarix kaempferi Gord and its influence on the glycogen and total protein of snails in Hubei,and identify it.Methods The molluscicidal efficacy of different concentration of endophyte JJ18 was detected according to the immersion test method recommended by WHO.The glycogen and protein of Oncomelania hupensis treated by endophyte JJ18 was determined by Anthrone and Kjeldahl method,respectively.JJ18 was identified by rDNA ITS sequence.Results The alcohol extract of JJ18 effectively killed O.hupensis.The mortality of O.hupensis was 100% after treatment with 400 mg/L alcohol extract for 3 d,which was similar to that treated with 1 mg/L niclosamidum for 2-3 d.Statistical analysis results revealed that the LC₅₀and LC₉₀ values of the alcohol extract of JJ18 were 145.0 mg/L and 311.9 mg/L after treatment for 72 h,respectively.The content of snail glycogen remarkably decreased after treatment with JJ18 extract,which ranged from 10.42% to 28.03%.The protein content did not significantly change,and JJ18 strain was Aspergillus niger after identification.Conclusion Endophyte JJ18 from P.kaempferi Gord is a new resource of molluscicide and has the development and application potential,which suggests that endophyte is a important resource to select new molluscicidal compounds.