关键词: 德国小蠊, 磷酸酯酶, 谷胱甘肽S-转移酶, 抗药性

Abstract: Objective To study the relationship between the activities of glutathione S-transferase(GSTs) and phosphatase from German cockroach(Blattella germanica) and its resistance,so as to reveal its resistance mechanism.Methods The activity of phosphatase and the values of the Michaelis-Menten constant(K_m) and the maximum velocity(V_max) were determined according to the method of Bessey et al.The activity of glutathione s-transferase and the values of its K_m and V_max were measured according to the methods of Clark,Kao and Booth.Results The activity of acidic phosphatase from sensitive strain and field strains were 0.98 and 26.95 nmol/(insect·30 min)respectively,and the specific activities of them were 1.45 and 1.59 μmol/(mg pro·30 min).The K_m values were 26.14 and 0.89 mmol/L,and the values of the V_max were 3.33 and 0.85 nmol/(mg pro·30 min)respectively.The activity of alkaline phosphatase(ALP) from two strains were(0.03±0.00) and(0.28±0.06)nmol/(insect·30 min),and their specific activities were(0.33±0.00) and(0.37±0.00)μmol/(mg pro·30 min) respectively.The values of the K_m were 70.38 and 61.24 mmol/L,and the values of the V_max were 16.20 and 14.00 nmol/(mg pro·30 min). The activity of GSTs from two strains were 0.13 and 0.47 nmol/min respectively,and the specific activities were 8.94 and 17.37 nmol/(mg pro·min) respectively.The values of the K_m were 2.08 and 5.81 mmol/L,and the values of the V_max were 0.08 and 0.17 nmol/min.Conclusion The activity change of glutathione s-transferase and phosphatase from field strain German cockroaches was related to the resistance.