关键词: 蜚蠊, 遗传多态性, 随机扩增DNA多态性, 基因组DNA

Abstract: Objective To investigate genetic polymorphism among four species of cockroaches in Hainan Island and find the molecular marker of cockroach classification.Methods Randomly amplified polymorphic DNA(RAPD)were used to amplify the DNA from Periplaneta americana,P.australasiae,P.fuliginosa and P.brunnea,and the RAPD results were used to compute genetic distances and draw genetic relationship clustering map.Results Four random primers were selected,and 122 electrophoresis bands with different molecular weight were amplified,71.31% of which were polymorphic bands.By analyses,the minimum genetic distance was 0.0345 between P.australasiae and P.fuliginosa,while the maximum was 0.1429 between P.americana and P.brunnea.In the phylogenetic tree,P.fuliginosa was clustered with P.australasiae firstly,and then clustered with P.brunnea,and P.americana.Conclusion The genome DNA of cockroach was highly polymorphic.And RAPD could be used to reflect the genetic relationship among cockroaches and classify accurately.