关键词: 家蝇蛹, 抗菌肽, 抗弓形虫, 纯化

Abstract: Objective Isolate and purify the antimicrobial peptides with anti-Toxoplasma activity from the haemlymph of Musca domestica pupa. Methods The antimicrobial peptide of M.domestica pupa were produced largely by infection and injury,which were isolated and purified by trituration, centrifugalization and column chromatography of AKTA^TM purifier. Then the antimicrobial peptides with anti-Toxoplasma activity were selected by haemacytometry and MTT colorimetric method. Results It was found that there was a ultraviolet absorption peak at 280 nm when retention volume was 9.4 ml through Resource S cationic exchange column chromatography, which had anti-Toxoplasma activity. This protein was collected and purified further with Superdex G75 gel column chromatography. And there were six ultraviolet absorption peaks at 280 nm through Superdex G75 gel column chromatography, and the sixth one had anti-Toxoplasma activity. Conclusion There was an antimicrobial peptide with anti-Toxoplasma activity in the haemlymph of M.domestica pupa.