关键词: 汉坦病毒, S基因, 原核细胞, 表达

Abstract: Objective To clone S gene from the lung of mice and to realize the expression of its protein in vitro. Methods The S genes of YZG-Changchun stains of Hantavirus were amplified by RT-PCR, and then the RT-PCR products were cloned into the pMD18-T vector. After sequencing analysis, the foreign gene in the recombinant plasmid was cut by restriction enzymes and cloned into the expression vector pET-28a. The recombinant plasmid was then transformed into E.coli Rosetta induced with IPTG. To detect the expression and the immunoreactive of exogenous protein in recombination plasmid by SDS-PAGE and Western-blot. Results The fusion protein was about 37% of the total proteins by gel thin scanner instrument CS-9000 and possessed favourable immunoreactive. Conclusion The gene of S protein can be expressed in a high efficiency in vitro by genetic engineering method, so it provides a good basis for further research on its function and the development of Hantavirus vaccine.