关键词: 沃尔巴克氏体, 德国小蠊, wsp基因

Abstract: Objective To detect and analyze wsp gene of Wolbachia symbiotic in Blattella germanica. Methods The fragments of the wsp gene of B.germanica was amplified by PCR with general wsp primers(81F, 691R). Target PCR product was purified, T-A cloned, sequenced and identified. The amplified fragment sequence was aligned with another published wsp genes. Results The presence of Wolbachia had been successfully detected in B.germanica, and the length of this gene is 317 bp. Multiple sequence alignment indicated that .germanica wsp gene was very similar to another eukaryotic gene in GenBank and shared more than 95% identity. Conclusion It shows that perhaps Wolbachia are bacteria widespread in B.germanica, which may lay foundation on the further study of correlativity between parthenogenesis of B.germanica and Molbachia.