关键词: 钉螺, 内生真菌, 杀螺作用, 金钱松, JJ18, 发酵工艺

Abstract: Objective To improve the molluscicidal effect by optimization of fermentation medium of endophyte JJ18 from Pseudolarix kaempferi Gord. Methods The medium composition and the cultivation conditions were optimized by orthogonal and single factor experiment. The immersion method suggested by WHO was applied to determine the effect and the main assessment index was mortality of the snails and biomass of fermantation liquid. Results This optimum culture medium included potato of 175 g/L, initial pH 6.0, glucose of 15 g/L, sucrose of 15 g/L, KH₂PO₄ of 0.5 mmol/L, the inoculum dose of 2%, the medium content of 50 ml, temperature of 30 ℃, incubation of 7 d and 120 r/min. The mortality of the snails was 100% with the optimum culture medium, which was increased by 16.7%. There exists a certain correlation between biomass of endophyte JJ18 and mortality. Conclusion The mortality is improved with optimization of fermentation techniques, so endophyte JJ18 of Pseudolarix kaempferi Gord has potential for molluscicidal application.