关键词: 肾综合征出血热, 双抗原夹心法ELISA, 血清学诊断

Abstract: Objective To establish a sensitive and specific method for detecting total antibodies in sera of hemorrhagic fever with renal syndrome(HFRS) patients and rodents by the double antigens sandwich ELISA. Methods The double antigens sandwich ELISA was established with coating antigen e1.3S from Hantavirus (HV), combining with recombinant antigen e6-119 labeled with HRP. The method was used to detect total antibodies in sera of HFRS patients and rodents, and the results were compared to that of indirect fluorescent assay(IFA). Results Five hundred and sixty-six samples (188 human sera and 378 rodents sera) were detected by using the double antigens sandwich ELISA and IFA. The total coincidence rate of the results by the two methods was 97.70%. Compared with IFA, the ELISA for the detection of total antibodies showed 97.54% in sensitivity and 97.87% in specificity. Conclusion The double antigens sandwich ELISA showed higher sensitivity and specificity for the detection of total antibodies, which can be applied in the large-scale epidemiologic survey.