中国媒介生物学及控制杂志

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16S rRNA序列分析法检测国境口岸截获德国小蠊携带病原菌初探

马晓光1;宋秀平2;高博3;姚李四1;刘起勇2;蔡亨中3;张乐1   

  1. 1中国检验检疫科学研究院卫生检疫研究所 北京100025;2中国疾病预防控制中心传染病预防控制所;3福建出入境检验检疫局
  • 出版日期:2007-06-20 发布日期:2007-06-20

Primary study on the pathogenic bacteria in Blattella germanica intercepted and captured in Chinese ports of Fujian province by 16S rRNA sequence

MA Xiao-guang; SONG Xiu-ping; GAO Bo; YAO Li-si; LIU Qi-yong; CAI Heng-zhong; ZHANG Le   

  1. Chinese Academy of Inspection and Quarantine, Beijing 100025, China
  • Online:2007-06-20 Published:2007-06-20

摘要: 目的 调查从国境口岸截获的德国小蠊携带病原菌情况。方法 用选择性培养基对细菌进行分离,用基于16SrRNA的PCR扩增、序列测定和比对方法进行鉴定。结果 检测出肺炎克雷伯氏菌、解鸟氨酸克雷伯菌、普通变形菌、威隆气单胞菌、粘质沙雷氏菌5种重要条件致病菌,其序列同源性超过98%,这些细菌可引起肺炎、脓胸、腹膜炎、脑膜炎、心内膜炎及败血症等,有的还具有致死性。结论 国境口岸截获的德国小蠊携带多种重要条件致病菌。

关键词: 国境口岸, 德国小蠊, 条件致病菌

Abstract: Objective To study the pathogenic bacteria of Blattella germanica intercepted and captured in ports of Fujian province. Methods Bacteria were separated with selected culture medium, and then identified by PCR amplification, sequence analysis of 16S rRNA gene fragments. Results Five species of conditional pathogenic bacteria were detected, they were Klebsiella pneumoniae, Klebsiella ornithinolytica, Proteus vulgaris, Aeromonas veronii and Serratia marcescens based on the sequence homology more than 98% comparison with the published sequences. Pneumonia, abscess, peritonitis, meningitis, endocarditis and blood poisoning could be caused by these pathogenic bacteria. And one of them was deathful. Conclusion There are many conditional pathogenic bacteria in Blattella germanica intercepted and captured in ports of Fujian province.