关键词: 等位基因特异性聚合酶链反应, 点突变, 炭疽芽胞杆菌

Abstract: Objective To evaluate the allele specific polymerase chain reaction(AS-PCR) method applied to screen point mutagenesis in lef gene of Bacillus anthracis.Methods AS-PCR amplification.Results The false positive rate of AS-PCR was high,its accuracy was not correlative to the base type at the 3′ end of primers,and was correlative to annealing temperature and template concentration.The Taq DNA polymerase with proofreading activity couldn't improve the false positive.Conclusion AS-PCR amplification could be affected by many factors and is not adapt to be a screen method for point mutagenesis.