关键词: 德国小蠊, 抗药性, 羧酸酯酶

Abstract: Objective To evaluate the resistance of Blattella germanica against common used insecticides in Nanchang,and to study the characteristic of carboxylesterase(CarE) in both field and susceptible population.Methods The surface contact method of WHO recommended for cockroach were used for B.germanica insecticides resistance test,and Van Asperen method were used for CarE activity.Results The resistance indexes of dichlorvos,popoxur,deltamethrin,beta cypermethrin were >122.3,1.1,1.4,5.3,4.9 respectively in field population comparing to susceptible strain.The specific activities of the resistant and sensitive strains were 68.7567 mmol/(mgpro·30 min),56.5069 mmol/(mgpro·30 min);The values of K_m were 0.4171 mmoL/L,0.4374 mmol/L;The values of V_max were 0.8525 nmol/(mgpro·min),0.4075 nmol/(mgpro·min) respectively.Conclusion B.germanica in Nanchang developed resistance to most of the tested insecticides but propoxur,and the resistance was associated with the changes of biochemical activities of the CarE.