关键词: 尖音库蚊复组, 抗性基因, 聚合酶链反应-限制性片段长度多态性, 分子分型

Abstract: Objective Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) assay was applied to molecular typing of resistance-associated esterase genes of Culex pipiens complex.Methods The primers specific to the conserved region of resistance-associated esterase genes of C.pipiens were designed.The genomic DNA was extracted from single mosquito.PCR-RFLP were used in molecular typing of resistance-associated esterase genes.Results Csp6Ⅰ digestion pattern of five fragments(70 bp,89 bp,147 bp,198 bp,347 bp) was noted for homozygous resistance-associated esterase-β1¹,and the pattern of four fragments(60 bp,147 bp,207 bp,437 bp) was noted for homozygous resistance-associated esterase-β2,and the patterm of five fragments was noted for heterozygous Est-β1¹/Est-β2,and the pattern of three fragments(147 bp,207 bp,506 bp) was noted for homozygous Est-βN.Conclusion PCR-RFLP is an effective,specific method for molecular typing of resistance-associated esterase genes of C.pipiens complex.