关键词: 家蝇幼虫, 凝胶层析, 抗真菌肽

Abstract: Objective To establish the Gel filtration method of isolating antifungal peptides from hemolymph of housefly larvae(Musca domestica).Methods The third instar larvae were stabbed with needle,then put in fungal suspend solution which contains Candida albicans for 24 hours. Isolate antifungal peptides from the hemolymph by heating,Gel filtration,solid phase extraction and HPLC;analyze the antifungal peptides and compare the isolating effect from Gel filtration with that from solid phase extraction by SDS-PAGE electrophoresis.Results The antifungal group isolated by Gel filtration is more steady than the antifungal group isolated by solid phase extraction;SDS-PAGE electrophoresis results shows that the protein molecular weight after Gel filtration is less than 14.4×10³;The unnecessary protein of the sample isolated by Gel filtration is less than that by solid phase extraction.Conclusion Housefly larvae can produce antifungal peptides in it's hemolymph by induction;The Gel filtration is a good method to isolate the antifungal peptide.